Keratinases: emerging trends in production and applications as novel multifunctional biocatalysts

TLDR: Techniques such as immobilization, optimization strategies, proteinengineering and DNA recombinant technology have been used to improve keratinases activities and stabilities therebywidening the scope for commercialization.

Abstract: Keratinases are proteolytic enzymes capable of degrading rigid and insoluble keratinous proteins present in skinand appendages. They are produced in the keratinous substrates such as feather, hair, wool, nail, horn and hoof bymicroorganisms. They are mostly serine proteases, although there are very few reports about metallokeratinases.Keratinases are active over wide range of conditions, and are useful in biorecycling of keratin wastes into feed andfertilizers. They also have potential applications in leather, cosmetic, textile, biomedical and detergent industries. Thepromising applications of keratinases also extend to energy generation and green synthesis of nanoparticles. Owingto their ubiquitous biotechnological applications, techniques such as immobilization, optimization strategies, proteinengineering and DNA recombinant technology have been used to improve their activities and stabilities therebywidening the scope for commercialization. This review chronicles recent trends in the production and multi-functionalapplications of keratinases.