Journal ArticleDOI
Laser Capture Microdissection
Michael R. Emmert-Buck,Robert F. Bonner,Paul D. Smith,Rodrigo F. Chuaqui,Zhengping Zhuang,Seth R. Goldstein,Rhonda A. Weiss,Lance A. Liotta +7 more
TLDR
Laser capture microdissection under direct microscopic visualization permits rapid one-step procurement of selected human cell populations from a section of complex, heterogeneous tissue.Abstract:
Laser capture microdissection (LCM) under direct microscopic visualization permits rapid one-step procurement of selected human cell populations from a section of complex, heterogeneous tissue. In this technique, a transparent thermoplastic film (ethylene vinyl acetate polymer) is applied to the surface of the tissue section on a standard glass histopathology slide; a carbon dioxide laser pulse then specifically activates the film above the cells of interest. Strong focal adhesion allows selective procurement of the targeted cells. Multiple examples of LCM transfer and tissue analysis, including polymerase chain reaction amplification of DNA and RNA, and enzyme recovery from transferred tissue are demonstrated.read more
Citations
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Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.
TL;DR: A snapshot of the state-of-the-art in real-time RT-PCR is provided and some of the problems associated with interpreting results that are numerical and lend themselves to statistical analysis, yet whose accuracy is significantly affected by reagent and operator variability are described.
Journal ArticleDOI
Genomics, gene expression and DNA arrays.
TL;DR: Measurements of gene expression and other applications of arrays embody much of what is implied by the term ‘genomics’; they are broad in scope, large in scale, and take advantage of all available sequence information for experimental design and data interpretation in pursuit of biological understanding.
Journal ArticleDOI
Physiological heterogeneity in biofilms.
TL;DR: The processes that generate chemical gradients inBiofilms, the genetic and physiological responses of the bacteria as they adapt to these gradients and the techniques that can be used to visualize and measure the microscale physiological heterogeneities of bacteria in biofilms are discussed.
Journal ArticleDOI
RNAscope: A Novel in Situ RNA Analysis Platform for Formalin-Fixed, Paraffin-Embedded Tissues
Fai Wang,John G. Flanagan,Nan Su,Li Chong Wang,Son Bui,Allissa Nielson,Xingyong Wu,Hong Thuy Vo,Xiao Jun Ma,Yuling Luo +9 more
TL;DR: RNAscope is described, a novel RNA ISH technology with a unique probe design strategy that allows simultaneous signal amplification and background suppression to achieve single-molecule visualization while preserving tissue morphology and may enable rapid development of RNAISH-based molecular diagnostic assays.
Journal ArticleDOI
Positional Cloning of the Gene for Multiple Endocrine Neoplasia-Type 1
Settara C. Chandrasekharappa,Siradanahalli C. Guru,Pachiappan Manickam,Shodimu Emmanuel Olufemi,Francis S. Collins,Michael R. Emmert-Buck,Larisa V. Debelenko,Zhengping Zhuang,Irina A. Lubensky,Lance A. Liotta,Judy S. Crabtree,Yingping Wang,Bruce A. Roe,Jane M. Weisemann,Mark S. Boguski,Sunita K. Agarwal,Mary Beth Kester,Young Sik Kim,Christina Heppner,Qihan Dong,Allen M. Spiegel,A. Lee Burns,Stephen J. Marx +22 more
TL;DR: The identification of MEN1 will enable improved understanding of the mechanism of endocrine tumorigenesis and should facilitate early diagnosis of multiple endocrine neoplasia-type 1.
References
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Intraductal dysplasia: A premalignant lesion of the prostate
John E. McNeal,David G. Bostwick +1 more
TL;DR: It was concluded that dysplasia is probably a direct biologic precursor of prostatic carcinoma and may be the antecedent lesion in the majority of prostatics cancers.
Journal ArticleDOI
Pulsed CO2 laser tissue ablation: Effect of tissue type and pulse duration on thermal damage
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Journal Article
Allelic Loss on Chromosome 8p12–21 in Microdissected Prostatic Intraepithelial Neoplasia
Michael R. Emmert-Buck,Cathy D. Vocke,Rudy Pozzatti,Paul H. Duray,Scott B. Jennings,Charles Florence,Zhengping Zhuang,David G. Bostwick,Lance A. Liotta,W. Marston Linehan +9 more
TL;DR: The results are consistent with the hypothesis that PIN arises multifocally within the prostate gland, and that a subset of these lesions progress to become carcinoma.