Journal ArticleDOI
Laser microdissection: A promising tool for exploring microorganisms and their interactions with hosts.
TLDR
The current paper describes the methodological aspects of commercially available laser microdissection instruments and representative examples that demonstrate the advantages of this method for resolving a variety of issues in microbiology.About:
This article is published in Journal of Microbiological Methods.The article was published on 2017-07-01. It has received 17 citations till now. The article focuses on the topics: Laser capture microdissection.read more
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One Bacterial Cell, One Complete Genome
TL;DR: In this article, the authors reported the completed genome from an uncultured single cell of Candidatus Sulcia muelleri DMIN, which is a polyploid species with genome copies ranging from approximately 200-900 per cell.
Journal ArticleDOI
Dual RNA-Sequencing to Elucidate the Plant-Pathogen Duel.
TL;DR: This review focuses on the design of dual RNA-seq experiments and the application of downstream data analysis to gain biological insight into both sides of the interaction and a reduction in sequencing cost and single cell transcriptomics coupled with protein and metabolite level dual approaches are set to enhance understanding of plant-pathogen interactions.
Journal ArticleDOI
Laser microdissection: A powerful tool for genomics at cell level.
TL;DR: How the latest developments in protocols and methods have made LM a powerful and sometimes essential tool for genomic and proteomic analyzes of tiny amounts of biomolecules extracted from few cells isolated from a complex tissue, in their physiological context, thus offering new opportunities for understanding fundamental physiological and/or patho-physiological processes is seen.
Journal ArticleDOI
Laser capture microdissection to study Bacillus cereus iron homeostasis gene expression during Galleria mellonella in vivo gut colonization.
Laurent Consentino,Agnès Rejasse,Nicolas Crapart,Claudia Bevilacqua,Christina Nielsen-LeRoux +4 more
TL;DR: In this paper, the authors used laser-capture microdissection (LCM) to select precise areas in the gut lumen from frozen whole larval cryo-sections.
Journal ArticleDOI
Microbiome analysis from formalin-fixed paraffin-embedded tissues: Current challenges and future perspectives.
TL;DR: In this article , the authors summarize all aspects of microbiome studies from FFPE tissues including the challenges associated with working highly degraded DNA, best practices for reducing environmental contamination, and propose solutions to address these issues.
References
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Journal ArticleDOI
Siboglinid-bacteria endosymbiosis: A model system for studying symbiotic mechanisms.
TL;DR: Ad adopting a comparative approach using different siboglinid groups, such as worms harboring thiotrophic versus methanotrophic endosymbionts, may yield considerable insight into the ecology and evolution of the Sibogliidae.
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Improved resolution by mounting of tissue sections for laser microdissection.
TL;DR: A mounting method that greatly improves the morphology of tissue sections for laser microbeam microdissection purposes and can be combined with various staining methods without compromising the quality of the DNA extracted is developed.
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Use of laser microdissection for phylogenetic characterization of polyphosphate-accumulating bacteria.
TL;DR: A novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis.
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Fungal species identification from avian lung specimens by single hypha laser microdissection and PCR product sequencing.
TL;DR: Using clinical specimens from infected birds, it is shown that the procedure is suitable for species identification from single hyphae of intralesional filamentous fungi and suggest that multiple Aspergillus fumigatus strain infections may occur frequently in pulmonary aspergillosis of birds.
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Live cell isolation by laser microdissection with gravity transfer.
TL;DR: Data obtained in this study confirm that LMDGT is a reliable and high-yield method allowing isolation and expansion of both cell clusters and single cells from adherent cell cultures.