Journal ArticleDOI
Laser microdissection: A promising tool for exploring microorganisms and their interactions with hosts.
TLDR
The current paper describes the methodological aspects of commercially available laser microdissection instruments and representative examples that demonstrate the advantages of this method for resolving a variety of issues in microbiology.About:
This article is published in Journal of Microbiological Methods.The article was published on 2017-07-01. It has received 17 citations till now. The article focuses on the topics: Laser capture microdissection.read more
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Journal Article
One Bacterial Cell, One Complete Genome
TL;DR: In this article, the authors reported the completed genome from an uncultured single cell of Candidatus Sulcia muelleri DMIN, which is a polyploid species with genome copies ranging from approximately 200-900 per cell.
Journal ArticleDOI
Dual RNA-Sequencing to Elucidate the Plant-Pathogen Duel.
TL;DR: This review focuses on the design of dual RNA-seq experiments and the application of downstream data analysis to gain biological insight into both sides of the interaction and a reduction in sequencing cost and single cell transcriptomics coupled with protein and metabolite level dual approaches are set to enhance understanding of plant-pathogen interactions.
Journal ArticleDOI
Laser microdissection: A powerful tool for genomics at cell level.
TL;DR: How the latest developments in protocols and methods have made LM a powerful and sometimes essential tool for genomic and proteomic analyzes of tiny amounts of biomolecules extracted from few cells isolated from a complex tissue, in their physiological context, thus offering new opportunities for understanding fundamental physiological and/or patho-physiological processes is seen.
Journal ArticleDOI
Laser capture microdissection to study Bacillus cereus iron homeostasis gene expression during Galleria mellonella in vivo gut colonization.
Laurent Consentino,Agnès Rejasse,Nicolas Crapart,Claudia Bevilacqua,Christina Nielsen-LeRoux +4 more
TL;DR: In this paper, the authors used laser-capture microdissection (LCM) to select precise areas in the gut lumen from frozen whole larval cryo-sections.
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Microbiome analysis from formalin-fixed paraffin-embedded tissues: Current challenges and future perspectives.
TL;DR: In this article , the authors summarize all aspects of microbiome studies from FFPE tissues including the challenges associated with working highly degraded DNA, best practices for reducing environmental contamination, and propose solutions to address these issues.
References
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Journal ArticleDOI
Sequential application of interphase-FISH and CGH to single cells
TL;DR: It is shown that the genome of individual cells can be analyzed by two different single cell techniques applied sequentially to the same cell, which represents the only option to control and verify the single-cell CGH results.
Journal ArticleDOI
Identification of a Novel, Invasive, Not-Yet-Cultivated Treponema sp. in the Large Intestine of Pigs by PCR Amplification of the 16S rRNA Gene
TL;DR: The spirochete, here named “Candidatus Treponema suis,” was associated with colitis, including invasion of the surface epithelium as well as superficial parts of the mucosa.
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Optimizing Staining Protocols for Laser Microdissection of Specific Cell Types from the Testis Including Carcinoma In Situ
Si Brask Sonne,Marlene Danner Dalgaard,John E. Nielsen,Christina E. Hoei-Hansen,Ewa Rajpert-De Meyts,Lise Mette Rahbek Gjerdrum,Henrik Leffers +6 more
TL;DR: Two staining protocols for frozen sections are presented, one for fast and specific staining of fetal germ cells, testicular carcinoma in situ cells, and other cells with embryonic stem cell-like properties that express the alkaline phosphatase, and one for specificStaining of lipid droplet-containing cells, which is useful for isolation of the androgen-producing Leydig cells.
Journal ArticleDOI
Laser capture microdissection for the investigative pathologist.
TL;DR: Key practical aspects in tissue handling, specimen selection, quality control, and sample preparation for LCM and downstream molecular analyses that are a primary objective of the investigative pathologist are examined.
Journal ArticleDOI
Laser microdissection: exploring host-bacterial encounters at the front lines.
TL;DR: Laser microdissection has already yielded key insights into the nature of mucosal responses to commensal, as well as pathogenic bacteria, and promises to be an important addition to the cellular microbiologist's toolkit.