Journal ArticleDOI
Laser microdissection: A promising tool for exploring microorganisms and their interactions with hosts.
TLDR
The current paper describes the methodological aspects of commercially available laser microdissection instruments and representative examples that demonstrate the advantages of this method for resolving a variety of issues in microbiology.About:
This article is published in Journal of Microbiological Methods.The article was published on 2017-07-01. It has received 17 citations till now. The article focuses on the topics: Laser capture microdissection.read more
Citations
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Journal Article
One Bacterial Cell, One Complete Genome
TL;DR: In this article, the authors reported the completed genome from an uncultured single cell of Candidatus Sulcia muelleri DMIN, which is a polyploid species with genome copies ranging from approximately 200-900 per cell.
Journal ArticleDOI
Dual RNA-Sequencing to Elucidate the Plant-Pathogen Duel.
TL;DR: This review focuses on the design of dual RNA-seq experiments and the application of downstream data analysis to gain biological insight into both sides of the interaction and a reduction in sequencing cost and single cell transcriptomics coupled with protein and metabolite level dual approaches are set to enhance understanding of plant-pathogen interactions.
Journal ArticleDOI
Laser microdissection: A powerful tool for genomics at cell level.
TL;DR: How the latest developments in protocols and methods have made LM a powerful and sometimes essential tool for genomic and proteomic analyzes of tiny amounts of biomolecules extracted from few cells isolated from a complex tissue, in their physiological context, thus offering new opportunities for understanding fundamental physiological and/or patho-physiological processes is seen.
Journal ArticleDOI
Laser capture microdissection to study Bacillus cereus iron homeostasis gene expression during Galleria mellonella in vivo gut colonization.
Laurent Consentino,Agnès Rejasse,Nicolas Crapart,Claudia Bevilacqua,Christina Nielsen-LeRoux +4 more
TL;DR: In this paper, the authors used laser-capture microdissection (LCM) to select precise areas in the gut lumen from frozen whole larval cryo-sections.
Journal ArticleDOI
Microbiome analysis from formalin-fixed paraffin-embedded tissues: Current challenges and future perspectives.
TL;DR: In this article , the authors summarize all aspects of microbiome studies from FFPE tissues including the challenges associated with working highly degraded DNA, best practices for reducing environmental contamination, and propose solutions to address these issues.
References
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Journal ArticleDOI
Gene expression in the tuberculous granuloma: analysis by laser capture microdissection and real-time PCR.
Guofeng Zhu,Huifang Xiao,Vellore P. Mohan,Kathryn E. Tanaka,Sanjay Tyagi,Fred Tsen,Padmini Salgame,John Chan +7 more
TL;DR: The results have provided evidence indicating that conventional use of whole infected lungs to study granuloma‐specific gene expression can yield data that may not genuinely reflect intralesional events, and suggest that the host immune system can adjust and respond to, or can be modulated by specific physiological states of the tubercle bacillus in vivo.
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Laser capture microdissection and metagenomic analysis of intact mucosa-associated microbial communities of human colon.
Yunwei Wang,Dionysios A. Antonopoulos,Dionysios A. Antonopoulos,Xiaorong Zhu,Laura Harrell,Ira M. Hanan,John C. Alverdy,Folker Meyer,Mark W. Musch,Vincent B. Young,Eugene B. Chang +10 more
TL;DR: The feasibility of laser capture microdissection of intact regional human colonic mucosa-associated microbes followed by phi29 multiple displacement amplification (MDA) and massively parallel sequencing for metagenomic analysis is determined.
Journal ArticleDOI
Laser capture microdissection: a novel approach to microanalysis of plant–microbe interactions
TL;DR: This review illustrates the application of LCM in studies on gene expression profiling and proteomics, and also in research on plant-microbe interactions.
Journal ArticleDOI
Laser-assisted microdissection for real-time PCR sample preparation.
TL;DR: Trying to highlight the advantages and disadvantages of LMD, the main problems related to specimens collection and processing, section preparation and extraction of bio-molecules from microdissected tissue samples have been analysed.
Journal Article
Beyond laser microdissection technology: follow the yellow brick road for cancer research.
TL;DR: The different applications of laser microdissection in a variety of fields are reviewed, and attention is focused on the pre-analytical steps that are crucial to successfully perform molecular-level investigations.