Journal ArticleDOI
Laser microdissection: A promising tool for exploring microorganisms and their interactions with hosts.
TLDR
The current paper describes the methodological aspects of commercially available laser microdissection instruments and representative examples that demonstrate the advantages of this method for resolving a variety of issues in microbiology.About:
This article is published in Journal of Microbiological Methods.The article was published on 2017-07-01. It has received 17 citations till now. The article focuses on the topics: Laser capture microdissection.read more
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Journal Article
One Bacterial Cell, One Complete Genome
TL;DR: In this article, the authors reported the completed genome from an uncultured single cell of Candidatus Sulcia muelleri DMIN, which is a polyploid species with genome copies ranging from approximately 200-900 per cell.
Journal ArticleDOI
Dual RNA-Sequencing to Elucidate the Plant-Pathogen Duel.
TL;DR: This review focuses on the design of dual RNA-seq experiments and the application of downstream data analysis to gain biological insight into both sides of the interaction and a reduction in sequencing cost and single cell transcriptomics coupled with protein and metabolite level dual approaches are set to enhance understanding of plant-pathogen interactions.
Journal ArticleDOI
Laser microdissection: A powerful tool for genomics at cell level.
TL;DR: How the latest developments in protocols and methods have made LM a powerful and sometimes essential tool for genomic and proteomic analyzes of tiny amounts of biomolecules extracted from few cells isolated from a complex tissue, in their physiological context, thus offering new opportunities for understanding fundamental physiological and/or patho-physiological processes is seen.
Journal ArticleDOI
Laser capture microdissection to study Bacillus cereus iron homeostasis gene expression during Galleria mellonella in vivo gut colonization.
Laurent Consentino,Agnès Rejasse,Nicolas Crapart,Claudia Bevilacqua,Christina Nielsen-LeRoux +4 more
TL;DR: In this paper, the authors used laser-capture microdissection (LCM) to select precise areas in the gut lumen from frozen whole larval cryo-sections.
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Microbiome analysis from formalin-fixed paraffin-embedded tissues: Current challenges and future perspectives.
TL;DR: In this article , the authors summarize all aspects of microbiome studies from FFPE tissues including the challenges associated with working highly degraded DNA, best practices for reducing environmental contamination, and propose solutions to address these issues.
References
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Journal ArticleDOI
Laser Microdissection: a Sample Preparation Technique for Plant Micrometabolic Profiling
Jingjing Fang,Bernd Schneider +1 more
TL;DR: Laser microdissection is a powerful sampling technique for plant micrometabolic profiling and metabolomics research and the wide application of this method for high-resolution plant metabolite analysis is indicated.
Journal ArticleDOI
Concurrent host-pathogen gene expression in the lungs of pigs challenged with Actinobacillus pleuropneumoniae
Louise Brogaard,Kirstine Klitgaard,Peter M. H. Heegaard,Mette Sif Hansen,Tim Kåre Jensen,Kerstin Skovgaard +5 more
TL;DR: This is the first study investigating the concurrent transcriptional response of both bacteria and host at the site of infection during porcine respiratory infection, and demonstrates the applicability of high-throughput RT-qPCR for the elucidation of dual-organism gene expression analysis during infection.
Journal ArticleDOI
Microdissection and the study of cancer pathways.
TL;DR: Improved techniques of tissue fixation and microdissection, supplemented with ancillary technology such as pre-amplification, have permitted the use of increasingly smaller quantities of material for the study of cancer pathways, and it is now possible to analyze many of the genetic changes that precede cancer, thereby identifying populations "at risk" for developing malignancies in the future.
Journal ArticleDOI
Influence of E. coli-induced prostatic inflammation on expression of androgen-responsive genes and transforming growth factor beta 1 cascade genes in rats.
Yasuhito Funahashi,Yasuhito Funahashi,Zhou Wang,Katherine J. O’Malley,Pradeep Tyagi,Donald B. DeFranco,Jeffrey R. Gingrich,Ryosuke Takahashi,Tsuyoshi Majima,Tsuyoshi Majima,Momokazu Gotoh,Naoki Yoshimura +11 more
TL;DR: This work has investigated the effects of prostatic inflammation on expression levels of androgen‐responsive genes and growth factors in the rat prostate and found that prostatic hyperplasia is associated with elevated levels of these genes and factors.
Journal ArticleDOI
Spatial manipulation of cells and organelles using single electrode dielectrophoresis.
TL;DR: A simple and low cost dielectrophoretic device for picking out and relocating single target cells and is capable of redistributing intracellular organelles is described.