Journal ArticleDOI
Laser microdissection: A promising tool for exploring microorganisms and their interactions with hosts.
TLDR
The current paper describes the methodological aspects of commercially available laser microdissection instruments and representative examples that demonstrate the advantages of this method for resolving a variety of issues in microbiology.About:
This article is published in Journal of Microbiological Methods.The article was published on 2017-07-01. It has received 17 citations till now. The article focuses on the topics: Laser capture microdissection.read more
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One Bacterial Cell, One Complete Genome
TL;DR: In this article, the authors reported the completed genome from an uncultured single cell of Candidatus Sulcia muelleri DMIN, which is a polyploid species with genome copies ranging from approximately 200-900 per cell.
Journal ArticleDOI
Dual RNA-Sequencing to Elucidate the Plant-Pathogen Duel.
TL;DR: This review focuses on the design of dual RNA-seq experiments and the application of downstream data analysis to gain biological insight into both sides of the interaction and a reduction in sequencing cost and single cell transcriptomics coupled with protein and metabolite level dual approaches are set to enhance understanding of plant-pathogen interactions.
Journal ArticleDOI
Laser microdissection: A powerful tool for genomics at cell level.
TL;DR: How the latest developments in protocols and methods have made LM a powerful and sometimes essential tool for genomic and proteomic analyzes of tiny amounts of biomolecules extracted from few cells isolated from a complex tissue, in their physiological context, thus offering new opportunities for understanding fundamental physiological and/or patho-physiological processes is seen.
Journal ArticleDOI
Laser capture microdissection to study Bacillus cereus iron homeostasis gene expression during Galleria mellonella in vivo gut colonization.
Laurent Consentino,Agnès Rejasse,Nicolas Crapart,Claudia Bevilacqua,Christina Nielsen-LeRoux +4 more
TL;DR: In this paper, the authors used laser-capture microdissection (LCM) to select precise areas in the gut lumen from frozen whole larval cryo-sections.
Journal ArticleDOI
Microbiome analysis from formalin-fixed paraffin-embedded tissues: Current challenges and future perspectives.
TL;DR: In this article , the authors summarize all aspects of microbiome studies from FFPE tissues including the challenges associated with working highly degraded DNA, best practices for reducing environmental contamination, and propose solutions to address these issues.
References
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Book
Laser-Tissue Interactions: Fundamentals and Applications
TL;DR: The physical fundamentals such as reflexion, absorption and scattering, effects such as heat generation and conduction, plasma induction, the formation of shock waves, and cavitation are treated in detail.
Journal ArticleDOI
Microfluidic sorting of mammalian cells by optical force switching.
Mark M. Wang,Eugene Tu,Daniel E. Raymond,Joon Mo Yang,Haichuan Zhang,Norbert Hagen,Bob Dees,Elinore M. Mercer,Anita H. Forster,Ilona Kariv,Philippe J. Marchand,William F. Butler +11 more
TL;DR: It is shown that optical forces can be used for the rapid (2–4 ms), active control of cell routing on a microfluidic chip and reduced complexity of the chip and simplify connectivity.
Journal ArticleDOI
The in vivo biofilm
Thomas Bjarnsholt,Thomas Bjarnsholt,Maria Alhede,Morten Alhede,Morten Alhede,Steffen Robert Eickhardt-Sørensen,Claus Moser,Michael Kühl,Michael Kühl,Michael Kühl,Peter Østrup Jensen,Niels Høiby,Niels Høiby +12 more
TL;DR: Why the current in vitro models of biofilms might be limited for describing infectious biofilmms are discussed, and new strategies for improving this discrepancy are suggested.
Journal ArticleDOI
Identification of expressed genes by laser-mediated manipulation of single cells
Karin Schütze,Georgia Lahr +1 more
TL;DR: This work describes a rapid noncontact method for the capture of single cells or small tissue areas of any size or shape directly within the cap of a common microfuge tube and was able to detect point mutations within codon 12 of c-Ki-ras2 mRNA after nested RT-PCR analysis.
Journal ArticleDOI
Laser-Capture Microdissection, a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types: Identification of Genes Expressed Differentially in Epidermal Cells or Vascular Tissues of Maize
TL;DR: The results demonstrate that the combination of LCM and microarrays makes it feasible to conduct high-resolution global gene expression analyses of plants and has the potential to enhance the understanding of diverse plant cell type-specific biological processes.