Journal ArticleDOI
Laser microdissection: A promising tool for exploring microorganisms and their interactions with hosts.
TLDR
The current paper describes the methodological aspects of commercially available laser microdissection instruments and representative examples that demonstrate the advantages of this method for resolving a variety of issues in microbiology.About:
This article is published in Journal of Microbiological Methods.The article was published on 2017-07-01. It has received 17 citations till now. The article focuses on the topics: Laser capture microdissection.read more
Citations
More filters
Journal Article
One Bacterial Cell, One Complete Genome
TL;DR: In this article, the authors reported the completed genome from an uncultured single cell of Candidatus Sulcia muelleri DMIN, which is a polyploid species with genome copies ranging from approximately 200-900 per cell.
Journal ArticleDOI
Dual RNA-Sequencing to Elucidate the Plant-Pathogen Duel.
TL;DR: This review focuses on the design of dual RNA-seq experiments and the application of downstream data analysis to gain biological insight into both sides of the interaction and a reduction in sequencing cost and single cell transcriptomics coupled with protein and metabolite level dual approaches are set to enhance understanding of plant-pathogen interactions.
Journal ArticleDOI
Laser microdissection: A powerful tool for genomics at cell level.
TL;DR: How the latest developments in protocols and methods have made LM a powerful and sometimes essential tool for genomic and proteomic analyzes of tiny amounts of biomolecules extracted from few cells isolated from a complex tissue, in their physiological context, thus offering new opportunities for understanding fundamental physiological and/or patho-physiological processes is seen.
Journal ArticleDOI
Laser capture microdissection to study Bacillus cereus iron homeostasis gene expression during Galleria mellonella in vivo gut colonization.
Laurent Consentino,Agnès Rejasse,Nicolas Crapart,Claudia Bevilacqua,Christina Nielsen-LeRoux +4 more
TL;DR: In this paper, the authors used laser-capture microdissection (LCM) to select precise areas in the gut lumen from frozen whole larval cryo-sections.
Journal ArticleDOI
Microbiome analysis from formalin-fixed paraffin-embedded tissues: Current challenges and future perspectives.
TL;DR: In this article , the authors summarize all aspects of microbiome studies from FFPE tissues including the challenges associated with working highly degraded DNA, best practices for reducing environmental contamination, and propose solutions to address these issues.
References
More filters
Journal ArticleDOI
Global Transcriptome Analysis of Genetically Identified Neurons in the Adult Cortex
Moritz J. Rossner,Johannes Hirrlinger,Sven P. Wichert,Christine Boehm,Dieter Newrzella,Holger Hiemisch,Gisela Eisenhardt,Carolin Stuenkel,Oliver von Ahsen,Klaus-Armin Nave +9 more
TL;DR: This analysis shows that cellularly complex targets lead to averaged gene expression profiles that lack substantial amounts of cell type-specific information in the CNS, andcell type-restricted sampling strategies are mandatory in the brains of transgenic mice.
Journal ArticleDOI
Plant single-cell and single-cell-type metabolomics.
TL;DR: Progress, current status, opportunities, and challenges presented by single cell-based metabolomics research in plants are reviewed.
Journal ArticleDOI
Probiotics stimulate enterocyte migration and microbial diversity in the neonatal mouse intestine
Geoffrey A. Preidis,Delphine M. Saulnier,Sarah E. Blutt,Toni-Ann Mistretta,Kevin Riehle,Angela Major,Susan Venable,Milton J. Finegold,Joseph F. Petrosino,Margaret E. Conner,James Versalovic +10 more
TL;DR: Two targets of probiosis in early development are identified, the intestinal epithelium and the gut microbiome, and suggest novel mechanisms for probiotic strain‐specific effects, which are identified and suggested in these experiments.
Journal ArticleDOI
A roadmap of cell-type specific gene expression during sequential stages of the arbuscular mycorrhiza symbiosis
Claudia Hogekamp,Helge Küster +1 more
TL;DR: A genome-wide analysis of transcription patterns in defined cell-types of Medicago truncatula roots mycorrhizal roots identified distinct groups of AM-activated genes governing the sequential reprogramming of host roots towards an accommodation of microsymbionts, including 42 AM-activated transcription factor genes.
Book ChapterDOI
Methods in cell separations.
TL;DR: Cell fractionation by size and density and highly selective affinity-based technologies such as affinity chromatography, fluorescence-activated cell sorting (FACS) and magnetic cell sorting are discussed in terms of throughput, yield, and purity.