Journal ArticleDOI
Lead-Induced Hypertension: Interplay of Nitric Oxide and Reactive Oxygen Species
Reads0
Chats0
TLDR
The data suggest a balance between increased NO synthesis and degradation (by reactive oxygen species) in lead-treated rats, which results in normal levels of NO.Abstract:
An elevation of mean blood pressure was found in rats treated with low lead (0.01% lead acetate) for 3 months, as contrasted to paired Sprague-Dawley control rats. In these rats, measurement of plasma and urine endothelins-1 and -3 revealed that plasma concentration and urinary excretion of endothelin-3 increased significantly after 3 months (plasma: lead group, 31.8+/-2.2, versus controls, 23.0+1.7 pg/mL, P<.001; urinary excretion: lead group, 46.6+11.7, versus controls, 35.6+6.7 pg/24 h, P<.05), whereas endothelin-1 was unaffected. Plasma and urinary nitric oxide (NO) and cyclic GMP concentrations were not significantly changed. However, assay of plasma and kidney cortex malondialdehyde by high-pressure liquid chromatography, as a measure of reactive oxygen species, was elevated in lead-treated rats compared with that in control rats (plasma: lead group, 4.74+1.27, versus controls, 2.14+.49 micromol/L, P<.001; kidney cortex: lead group, 28.75+3.46, versus controls, 16.38+2.37 nmol/g wet weight, P<.001). There was increased NO synthase activity in lead-treated rat brain cortex and cerebellum. In lead-treated rat kidney cortex, the endothelial constitutive NO synthase protein mass was unaffected, whereas the inducible NO synthase protein mass was increased. These data suggest a balance between increased NO synthesis and degradation (by reactive oxygen species) in lead-treated rats, which results in normal levels of NO. Thus, the hypertension may be related to an increase in the pressure substances, endothelin-3 and reactive oxygen species, rather than to an absolute decrease in nitric NO.read more
Citations
More filters
Journal ArticleDOI
Nitric Oxide and Peroxynitrite in Health and Disease
TL;DR: Current evidence indicates that most of the cytotoxicity attributed to NO is rather due to peroxynitrite, produced from the diffusion-controlled reaction between NO and another free radical, the superoxide anion, which is presented in detail in this review.
Toxicological Profile for Lead
Henry Abadin,Annette Ashizawa,Yee-Wan Stevens,Fernando Llados,Gary Diamond,Gloria Sage,Mario Citra,Antonio Quinones,Stephen J Bosch,Steven G Swarts +9 more
Journal ArticleDOI
Heavy Metals and Human Health: Mechanistic Insight into Toxicity and Counter Defense System of Antioxidants
TL;DR: A deep understanding of the mechanisms involved in eliciting heavy metals toxicity is provided in order to highlight the necessity for development of strategies to decrease exposure to these metals, as well as to identify substances that contribute significantly to overcome their hazardous effects within the body of living organisms.
Journal ArticleDOI
Aldosterone-Induced Inflammation in the Rat Heart: Role of Oxidative Stress
TL;DR: In this paper, aldosterone (ALDO) was shown to induce a proinflammatory/fibrogenic phenotype in both right and left ventricles in response to ALDO/salt treatment and that would be sustained with chronic treatment.
Can antioxidant be beneficial in the treatment of lead poisoning
TL;DR: In this paper, the importance of using antioxidants in treating lead poisoning was discussed, and the possible protective effects of antioxidants in lead toxicity were investigated. But, the authors did not consider the effect of antioxidant supplementation following lead exposure.
References
More filters
Journal ArticleDOI
Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
TL;DR: NO released from endothelial cells is indistinguishable from EDRF in terms of biological activity, stability, and susceptibility to an inhibitor and to a potentiator.
Journal ArticleDOI
A novel potent vasoconstrictor peptide produced by vascular endothelial cells.
Masashi Yanagisawa,Hiroki Kurihara,Hiroki Kurihara,Sadao Kimura,Yoko Tomobe,Mieko Kobayashi,Youji Mitsui,Yoshio Yazaki,Katsutoshi Goto,Tomoh Masaki +9 more
TL;DR: Cloning and sequencing of preproendothelin complementary DNA shows that mature endothelin is generated through an unusual proteolytic processing, and regional homologies to a group of neurotoxins suggest that endothelins is an endogenous modulator of voltage-dependent ion channels.
Journal ArticleDOI
Vascular endothelial cells synthesize nitric oxide from L-arginine.
TL;DR: It is demonstrated that NO can be synthesized from L-arginine by porcine aortic endothelial cells in culture and the strict substrate specificity of this reaction suggests that L- arginine is the precursor for NO synthesis in vascular endothelium cells.
Journal ArticleDOI
Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor
TL;DR: It is demonstrated that EDRF is protected from breakdown by superoxide dismutase (SOD) and Cu2+, but not by catalase, and is inactivated by Fe2+.