Journal ArticleDOI
Location of Diphenylhexatriene (DPH) and Its Derivatives within Membranes: Comparison of Different Fluorescence Quenching Analyses of Membrane Depth†
Robert D. Kaiser,Erwin London +1 more
TLDR
The average membrane location of a series of diphenylhexatriene (DPH)-derived membrane probes was analyzed by measuring the quenching of DPH fluorescence with aseries of nitroxide-labeled lipids.Abstract:
The average membrane location of a series of diphenylhexatriene (DPH)-derived membrane probes was analyzed by measuring the quenching of DPH fluorescence with a series of nitroxide-labeled lipids i...read more
Citations
More filters
Journal ArticleDOI
Fluorescent Analogs of Biomolecular Building Blocks: Design, Properties, and Applications
TL;DR: This article focuses on designing fluorescent probes for the four major families of macromolecular building blocks discussed above, and discusses emissive carbohydrate derivatives, followed byEmissive amino acids, the building blocks of nucleic acids.
Journal ArticleDOI
Role of cholesterol in the function and organization of G-protein coupled receptors.
TL;DR: A novel mechanism by which membrane cholesterol could affect structure and function of GPCRs is proposed and it is envisaged that there could be specific/nonannular cholesterol binding site(s) in the serotonin(1A) receptor.
Journal ArticleDOI
The relationship between the antioxidant and the antibacterial properties of galloylated catechins and the structure of phospholipid model membranes
TL;DR: It is proposed that the high antioxidant capacity of some galloylated catechins such as ECG could be partially due to the formation of membrane structures showing resistance to detergent solubilization and in which the phospholipids have tightly packed acyl chains and highly hydrated phosphate groups.
Journal ArticleDOI
Cholesterol modulates ligand binding and G-protein coupling to serotonin1A receptors from bovine hippocampus
TL;DR: Evidence is provided, for the first time, that cholesterol is necessary for ligand binding and G-protein coupling of this important neurotransmitter receptor and could have significant implications in understanding the influence of the membrane lipid environment on the activity and signal transduction of other G- protein-coupled transmembrane receptors.
Journal ArticleDOI
Phosphatidylethanolamine Is a Key Regulator of Membrane Fluidity in Eukaryotic Cells
Rosie Dawaliby,Cataldo Trubbia,Cédric Delporte,Caroline Noyon,Jean Marie Ruysschaert,Pierre Van Antwerpen,Cédric Govaerts +6 more
TL;DR: It is shown here that insect and mammalian cells present similar microviscosity at their respective physiological temperature, indicating that both cell types combine regulation by sterols and phospholipids to control proper membrane fluidity.
References
More filters
Journal ArticleDOI
Parallax method for direct measurement of membrane penetration depth utilizing fluorescence quenching by spin-labeled phospholipids.
TL;DR: The method involves determination of the parallax in the apparent location of fluorophores detected when quenching by phospholipids spin-labeled at two different depths and it is shown that the calculated depths of the NBD groups are self-consistent no matter which two spin-labels have been used in a particular experiment.
Journal ArticleDOI
Use of fluorescent probes to monitor molecular order and motions within liposome bilayers
TL;DR: This article reviews the use of fluorescent probes to monitor the order and dynamics within the acyl chain region of liposome lipid bilayers and the non-linear probes considered include the anthroyloxyl fatty acids and the recently introduced fluorenyl fatty acid probes.
Journal ArticleDOI
Relationships between lipid membrane area, hydrophobic thickness, and acyl-chain orientational order. The effects of cholesterol
TL;DR: A microscopic interaction model for a fully hydrated lipid bilayer membrane containing cholesterol is used to calculate the membrane area, the membrane hydrophobic thickness, and the average acyl-chain orientational order parameter, S, and it is pointed out that S under certain circumstances may be useful for estimating thehydrophobic membrane thickness.
Journal ArticleDOI
The Planar Organization of Lecithin-Cholesterol Bilayers
TL;DR: A model in which each cholesterol is surrounded by lipid hydrocarbon chains to describe the structure of the mixed phase of lecithin and cholesterol is proposed.
Journal ArticleDOI
Intramolecular quenching of excited singlet states by stable nitroxyl radicals
TL;DR: In this article, the authors examined the mechanism of excited singlet state quenching by nitroxides in a series of nitroxide-fluorophore adducts.