Journal ArticleDOI
Low Molecular Weight High–Mannose Type Glycans in a Secreted Protein of the Filamentous Fungus Trichoderma Reesei
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TLDR
The normal glycosylation pattern of CBH I, the major glycoprotein secreted by Trichoderma reesei, has been characterized and it may be possible to use T.Reesei as a host for the production of heterologous glycoproteins of animal origin, and to take advantage of its remarkable secretory capacity.Abstract:
With the object of obtaining information about the suitability of filamentous fungi for production of heterogeneous mammalian proteins, we have characterized the normal glycosylation pattern of CBH I, the major glycoprotein secreted by Trichoderma reesei. The protein–bound glycans of CBH I were studied using a variety of separation techniques to analyze the products of specific glycosidase digestions and chemical degradation procedures. CBH I contains both O–glycosidic and N–glycosidic glycans. The O–glycosidic glycans consist of one to four hexose residues which are mostly mannose. The structures of the N–glycosidic glycans are (Man)5(GlcNAc)2 and (Man)9(GlcNAc)2. Thus it may be possible to use T. reesei as a host for the production of heterologous glycoproteins of animal origin, and to take advantage of its remarkable secretory capacity.read more
Citations
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Journal ArticleDOI
Production of recombinant proteins by microbes and higher organisms.
Arnold L. Demain,Preeti Vaishnav +1 more
TL;DR: The most popular system for producing recombinant mammalian glycosylated proteins is that of mammalian cells while transgenic plants such as Arabidopsis thaliana and others can generate many recombinant proteins.
Journal ArticleDOI
Three-Dimensional Structure of Cellobiohydrolase II from Trichoderma reesei
TL;DR: The three-dimensional structure of a cellulase, the enzymatic core of CBHII from the fungus Trichoderma reesei reveals an alpha-beta protein with a fold similar to but different from the widely occurring barrel topology first observed in triose phosphate isomerase.
Book ChapterDOI
Microorganisms and enzymes involved in the degradation of plant fiber cell walls
TL;DR: This chapter describes the structure of wood and the main wood components, cellulose, hemicelluloses and lignins and the enzyme and enzyme mechanisms used by fungi and bacteria to modify and degrade these components are described in detail.
Patent
Methods for producing modified glycoproteins
TL;DR: In this article, a combination of engineering and/or selection of strains which do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved.
Journal ArticleDOI
The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain.
TL;DR: There is evidence for a correlation between the stability of transformants and integration of the vector in the genome whereas unstable transformants are obtained when autonomous replication of the plasmid occurs.
References
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Journal Article
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Journal ArticleDOI
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J Tilburn,Claudio Scazzocchio,Graham G. Taylor,J H Zabicky-Zissman,Robin A. Lockington,R.W. Davies +5 more
TL;DR: DNA-mediated genetic transformation of Aspergillus nidulans has been achieved by incubating protoplasts from a strain of A. niduans carrying a deletion in the acetamidase structural gene with DNA of derivatives of plasmid pBR322 containing the cloned structural gene for acetamids.