Magnesium-mediated Conversion of an Inactive Form of a Hammerhead Ribozyme to an Active Complex with Its Substrate AN INVESTIGATION BY NMR SPECTROSCOPY
Masaya Orita,Ravi Vinayak,Alex Andrus,Masaki Warashina,Masaki Warashina,Atsushi Chiba,Hidetoshi Kaniwa,Fumiko Nishikawa,Satoshi Nishikawa,Kazunari Taira,Kazunari Taira +10 more
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TLDR
In this paper, the effects of magnesium ions on a 32-mer ribozyme (R32) were examined by high resolution NMR spectroscopy in the presence of Mg2+ ions.About:
This article is published in Journal of Biological Chemistry.The article was published on 1996-04-19 and is currently open access. It has received 25 citations till now. The article focuses on the topics: Hairpin ribozyme & Hammerhead ribozyme.read more
Citations
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SURVEY AND SUMMARY Recent advances in the elucidation of the mechanisms of action of ribozymes
TL;DR: While high concentrations of monovalent metal ions or polyamines can facilitate cleavage by hammerhead ribozymes, divalent metal ions are the most effective acid/base catalysts under physiological conditions.
Journal ArticleDOI
Gene therapy for chronic viral hepatitis: ribozymes, antisense oligonucleotides, and dominant negative mutants.
F. von Weizsäcker,Stefan Wieland,Josef Köck,W.-B. Offensperger,S Offensperger,Darius Moradpour,Hubert E. Blum +6 more
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Formation of a catalytically active dimer by tRNA(Val)-driven short ribozymes.
Tomoko Kuwabara,Masaki Warashina,Masaki Warashina,Masaya Orita,Shiori Koseki,Shiori Koseki,Jun Ohkawa,Jun Ohkawa,Kazunari Taira,Kazunari Taira +9 more
TL;DR: The use of dimeric minizyme as gene-inactivating agents by placing minizymes under the control of a tRNAVal promoter inhibited reporter gene activity and resulted in 55% inhibition of hammerhead ribozyme activity.
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Hammerhead ribozyme design and application.
M Amarzguioui,H Prydz +1 more
TL;DR: This review addresses the design and application of hammerhead ribozymes, two aspects of a conserved and most commonly studied and used enzymatically active entity among the RNA enzymes.
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Effects of helical structures formed by the binding arms of DNAzymes and their substrates on catalytic activity
Naruhisa Ota,Masaki Warashina,Masaki Warashina,Kenichi Hirano,Kenichi Hatanaka,Kazunari Taira,Kazunari Taira +6 more
TL;DR: The reaction mechanism of metal-ion-dependent DNAzymes is almost identical to that of hammerhead ribozymes, suggesting that the structure-function relationships in reactions catalyzed by deoxyribozymes are clarified.
References
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Codon--anticodon pairing: the wobble hypothesis.
TL;DR: In this paper, it is suggested that while the standard base pairs may be used rather strictly in the first two positions of the triplet, there may be some wobble in the pairing of the third base.
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Simple RNA enzymes with new and highly specific endoribonuclease activities
Jim Haseloff,W. L. Gerlach +1 more
TL;DR: In vitro mutagenesis of sequences required for the self-catalysed cleavage of a plant virus satellite RNA has allowed definition of an RNA segment with endoribonuclease activity.
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A general two-metal-ion mechanism for catalytic RNA
Thomas A. Steitz,Joan A. Steitz +1 more
TL;DR: The mechanism postulates that chemical catalysis is facilitated by two divalent metal ions 3.9 A apart, as in phosphoryl transfer reactions catalyzed by protein enzymes, such as the 3',5'-exonuclease of Escherichia coli DNA polymerase I.
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Three-dimensional structure of a hammerhead ribozyme
TL;DR: The X-ray crystallographic structure of a hammerhead RNADNA ribozyme-inhibitor complex at 2.6 Å resolution reveals that the base-paired stems are A-form helices and the core has two structural domains.
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The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage.
TL;DR: The crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage has been solved and a mechanism for RNA catalytic cleavage is proposed.
Related Papers (5)
Simple RNA enzymes with new and highly specific endoribonuclease activities
Jim Haseloff,W. L. Gerlach +1 more