Mannitol 1-phosphate metabolism is required for sporulation in planta of the wheat pathogen Stagonospora nodorum.
TLDR
An expressed sequence tag encoding a putative mannitol 1-phosphate dehydrogenase (Mpd1) has been characterized from the fungal wheat pathogen Stagonospora nodorum, suggesting a role in metabolic and redox regulation during the critical process of sporulation on senescing leaf material.Abstract:
An expressed sequence tag encoding a putative mannitol 1-phosphate dehydrogenase (Mpd1) has been characterized from the fungal wheat pathogen Stagonospora nodorum. Mpd1 was disrupted by insertional mutagenesis, and the resulting mpd1 strains lacked all detectable NAD-linked mannitol 1-phosphate dehydrogenase activity (EC 1.1.1.17). The growth rates, sporulation, and spore viability of the mutant strains in vitro were not significantly different from the wild type. The viability of the mpd1 spores when subjected to heat stress was comparable to wild type. Characterization of the sugar alcohol content by nuclear magnetic resonance spectroscopy revealed that, when grown on glucose, the mutant strains contained significantly less mannitol, less arabitol, but more trehalose than the wildtype strains. The mannitol content of fructose-grown cultures was normal. No secreted mannitol could be detected in wild type or mutants. Pathogenicity assays revealed the disruption of Mpd1 did not affect lesion development, however the mutants were unable to sporulate. These results throw new light on the role of mannitol in fungal plant interactions, suggesting a role in metabolic and redox regulation during the critical process of sporulation on senescing leaf material.read more
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Transcriptome and metabolite profiling of the infection cycle of Zymoseptoria tritici on wheat reveals a biphasic interaction with plant immunity involving differential pathogen chromosomal contributions and a variation on the hemibiotrophic lifestyle definition.
Jason J. Rudd,Kostya Kanyuka,Keywan Hassani-Pak,Mark C. Derbyshire,Ambrose Andongabo,Jean Devonshire,Artem Lysenko,Mansoor Saqi,Nalini M. Desai,Stephen J. Powers,Juliet Hooper,Linda Ambroso,Arvind K. Bharti,Andrew Farmer,Kim E. Hammond-Kosack,Robert A. Dietrich,Mikael Courbot +16 more
TL;DR: The pathogenic lifestyle of Z. tritici on wheat revealed, involving initial defense suppression by a slow-growing extracellular and nutritionally limited pathogen followed by defense activation during reproduction, reveals a subtle modification of the conceptual definition of hemibiotrophic plant infection.
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Nutrition acquisition strategies during fungal infection of plants.
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Ecological metabolomics: overview of current developments and future challenges
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Stagonospora nodorum: From Pathology to Genomics and Host Resistance
TL;DR: Stagonospora nodorum is a major necrotrophic pathogen of wheat that causes the diseases S. nodorum leaf and glume blotch and has emerged as a model for the Pleosporales group of pathogens.
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Stagonospora nodorum: cause of stagonospora nodorum blotch of wheat
TL;DR: This review summarizes recent advances in the understanding of taxonomy, control and pathogenicity of this species, causing both a leaf and glume blotch of wheat.
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