Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass
Janice Zale,Je Hyeong Jung,Jae Yoon Kim,Bhuvan Pathak,Ratna Karan,Hui Liu,Xiuhua Chen,Hao Wu,Jason Candreva,Zhiyang Zhai,John Shanklin,Fredy Altpeter +11 more
TLDR
These results provide the basis for optimizations of TAG accumulation in sugarcane and other high yielding biomass grasses and will open new prospects for biofuel applications.Abstract:
Elevating the lipid content in vegetative tissues has emerged as a new strategy for increasing energy density and biofuel yield of crops. Storage lipids in contrast to structural and signaling lipids are mainly composed of glycerol esters of fatty acids, also known as triacylglycerol (TAG). TAGs are one of the most energy-rich and abundant forms of reduced carbon available in nature. Therefore, altering the carbon-partitioning balance in favour of TAG in vegetative tissues of sugarcane, one of the highest yielding biomass crops, is expected to drastically increase energy yields. Here we report metabolic engineering to elevate TAG accumulation in vegetative tissues of sugarcane. Constitutive co-expression of WRINKLED1 (WRI1), diacylglycerol acyltransferase1-2 (DGAT1-2) and oleosin1 (OLE1) and simultaneous cosuppression of ADP-glucose pyrophosphorylase (AGPase) and a subunit of the peroxisomal ABC transporter1 (PXA1) in transgenic sugarcane elevated TAG accumulation in leaves or stems by 95- or 43-fold to 1.9% or 0.9% of dry weight (DW), respectively, while expression or suppression of one to three of the target genes increased TAG levels by 1.5- to 9.5-fold. Accumulation of TAG in vegetative progeny plants was consistent with the results from primary transgenics and contributed to a total fatty acid content of up to 4.7% or 1.7% of DW in mature leaves or stems, respectively. Lipid droplets were visible within mesophyll cells of transgenic leaves by confocal fluorescence microscopy. These results provide the basis for optimizations of TAG accumulation in sugarcane and other high yielding biomass grasses and will open new prospects for biofuel applications.read more
Citations
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Journal ArticleDOI
Triacylglycerol Metabolism, Function, and Accumulation in Plant Vegetative Tissues.
Changcheng Xu,John Shanklin +1 more
TL;DR: Recent progress in the understanding ofTriacylglycerol synthesis, turnover, storage, and function in leaves is reviewed and emerging genetic engineering strategies targeted at enhancing triacyl glycerol accumulation in biomass crops are discussed.
Journal ArticleDOI
Plant Lipid Droplets and Their Associated Proteins: Potential for Rapid Advances.
TL;DR: Cytoplasmic lipid droplets and their associated oleosin and other proteins have evolved and diversified in functions and cell species specificities and have been probed for commercial uses.
Journal ArticleDOI
Lipid Droplet-Associated Proteins (LDAPs) Are Required for the Dynamic Regulation of Neutral Lipid Compartmentation in Plant Cells.
Satinder K. Gidda,Sunjung Park,Michal Pyc,Olga Yurchenko,Yingqi Cai,Peng Wu,David W. Andrews,Kent D. Chapman,John M. Dyer,Robert T. Mullen +9 more
TL;DR: Characterization of three plant-specific, lipid droplet-associated proteins in Arabidopsis revealed that LDAPs are required for the maintenance and regulation of LDs in plant cells and perform nonredundant functions in various physiological contexts, including stress response and postgerminative growth.
Journal ArticleDOI
Abiotic factors influence plant storage lipid accumulation and composition
TL;DR: The demand for plant-derived oils has increased substantially over the last decade, and is sure to keep growing, so it is clear that developing superior oil crops with the capability to perform in a consistent manner in field conditions in the future will be of the utmost importance.
Journal ArticleDOI
Step changes in leaf oil accumulation via iterative metabolic engineering
Thomas Vanhercke,Uday K. Divi,Anna El Tahchy,Qing Liu,Madeline Mitchell,Matthew C. Taylor,Peter J. Eastmond,Fiona M. Bryant,Anna Mechanicos,Cheryl Blundell,Yao Zhi,Srinivas Belide,Pushkar Shrestha,Xue-Rong Zhou,Jean-Philippe Ral,Rosemary G. White,Allan Green,Surinder P. Singh,James Robertson Petrie +18 more
TL;DR: The results demonstrate that the combined optimization of de novo fatty acid biosynthesis, storage lipid assembly and lipid turnover in leaf tissue results in a major overhaul of the plant central carbon allocation and lipid metabolism.
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