Metabolic remodeling of the human red blood cell membrane
YongKeun Park,Catherine A. Best,Thorsten Auth,Nir S. Gov,Samuel A. Safran,Gabriel Popescu,Subra Suresh,Michael S. Feld +7 more
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TLDR
It is presented conclusive evidence that the presence of adenosine 5′-triphosphate (ATP) facilitates non-equilibrium dynamic fluctuations in the RBC membrane that are highly correlated with the biconcave shape of RBCs.Abstract:
The remarkable deformability of the human red blood cell (RBC) results from the coupled dynamic response of the phospholipid bilayer and the spectrin molecular network. Here we present quantitative connections between spectrin morphology and membrane fluctuations of human RBCs by using dynamic full-field laser interferometry techniques. We present conclusive evidence that the presence of adenosine 5′-triphosphate (ATP) facilitates non-equilibrium dynamic fluctuations in the RBC membrane that are highly correlated with the biconcave shape of RBCs. Spatial analysis of the fluctuations reveals that these non-equilibrium membrane vibrations are enhanced at the scale of spectrin mesh size. Our results indicate that the dynamic remodeling of the coupled membranes powered by ATP results in non-equilibrium membrane fluctuations manifesting from both metabolic and thermal energies and also maintains the biconcave shape of RBCs.read more
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Quantitative phase imaging techniques for the study of cell pathophysiology: from principles to applications.
KyeoReh Lee,Kyoohyun Kim,JaeHwang Jung,Ji Han Heo,Sangyeon Cho,SangYun Lee,Gyuyoung Chang,YoungJu Jo,HyunJoo Park,YongKeun Park +9 more
TL;DR: The principles of QPI are presented and some of the recent applications ranging from cell homeostasis to infectious diseases and cancer are highlighted, to provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology.
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Diffraction phase microscopy: principles and applications in materials and life sciences
Basanta Bhaduri,Chris Edwards,Hoa V. Pham,Renjie Zhou,Tan H. Nguyen,Lynford L. Goddard,Gabriel Popescu +6 more
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Biomechanical properties of red blood cells in health and disease towards microfluidics.
TL;DR: The aim of this review is to summarize currently available reports on RBC deformability and to highlight its association with various human diseases such as hereditary disorders, metabolic disorders, adenosine triphosphate-induced membrane changes, oxidative stress, and paroxysmal nocturnal hemoglobinuria.
References
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YongKeun Park,Monica Diez-Silva,Gabriel Popescu,George Lykotrafitis,Wonshik Choi,Michael S. Feld,Subra Suresh +6 more
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