Journal ArticleDOI
Metabolism of trinitrobenzene sulfonic acid by the rat colon produces reactive oxygen species
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It was found that the addition of TNBS (1 mmol/L) to the 10,000 x g supernatant of rat colon homogenates increased the rate of superoxide production from normally undetectable levels to 2.6 +/- 0.23 nmol, suggesting that TNBS was required for the enhanced oxidative metabolism.About:
This article is published in Gastroenterology.The article was published on 1991-08-01. It has received 136 citations till now. The article focuses on the topics: Reactive oxygen species & Superoxide dismutase.read more
Citations
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Journal ArticleDOI
A comparative analysis of two models of colitis in rats.
TL;DR: Data obtained show that the inflammation induced by these four irritants results from caustic injury to the colonic epithelium and interstitium as measured by the rapid and dramatic increases in mucosal permeability and tissue water content as well as by histological analysis.
Journal ArticleDOI
Impairment of intestinal glutathione synthesis in patients with inflammatory bowel disease.
TL;DR: Investigation of constituent amino acid plasma levels and the GSH redox status in different compartments in IBD with emphasis on intestinal GSH synthesis in Crohn’s disease found reduced rGSH levels and increased GSSG levels were demonstrated in non-inflamed mucosa in patients with IBD.
Journal ArticleDOI
Mucosal injury and inflammation in a model of chronic granulomatous colitis in rats
TL;DR: PG-PS produces a chronic granulomatous colitis in rats; this colitis is characterized by enhanced NO production.
Journal ArticleDOI
Free radicals and other reactive oxygen metabolites in inflammatory bowel disease: cause, consequence or epiphenomenon?
Mary L. Harris,Henry J. Schiller,Patrick M. Reilly,Mark Donowitz,Matthew B. Grisham,Gregory B. Bulkley +5 more
TL;DR: In considering the therapeutic potential of free radical ablation for the treatment of patients with IBD it is important to distinguish between interventions that might specifically block the fundamental injury mechanism from those which would act in a more nonspecific, anti-inflammatory role.
Journal ArticleDOI
Superoxide dismutase ameliorates TNBS-induced colitis by reducing oxidative stress, adhesion molecule expression, and leukocyte recruitment into the inflamed intestine
Joaquim Seguí,Meritxell Gironella,Miquel Sans,Susana Granell,Felix Gil,Mercedes Gimeno,Pilar Coronel,Josep M. Piqué,Julián Panés +8 more
TL;DR: Treatment with SOD significantly reduces peroxidation reactions in the inflamed colon and affords significant amelioration of colonic inflammatory changes in experimental colitis.
References
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Journal ArticleDOI
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.
Book ChapterDOI
[59] Glutathione reductase
Inger Carlberg,Bengt Mannervik +1 more
TL;DR: A purification method of glutathione reductase from calf liver and rat liver is described and it is shown that this enzyme has a major role as a reductant in oxidation–reduction processes, and serves in detoxication and several other cellular functions of great importance.
Journal ArticleDOI
Hapten-Induced Model of Chronic Inflammation and Ulceration in the Rat Colon
Gerald P. Morris,Paul L. Beck,Margaret S. Herridge,William T. Depew,Myron R. Szewczuk,John L. Wallace +5 more
TL;DR: A simple and reproducible rat model of chronic colonic inflammation by the intraluminal instillation of a solution containing a "barrier breaker" and a hapten to study the pathophysiology of colonic inflammatory disease in a specifically controlled fashion and to evaluate new treatments potentially applicable to inflammatory bowel disease in humans.
Journal ArticleDOI
Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury
TL;DR: Evidence is presented to support the following hypothesis regarding the roles of xanthine oxidase-derived oxidants and granulocytes in ischemia-reperfusion-induced microvascular injury.
Book ChapterDOI
Preparation and assay of superoxide dismutases.
TL;DR: This chapter discusses the preparation and assay of superoxide dismutase (SOD), which is found in all oxygen-utilizing organisms and constitute a defense against oxygen toxicity.
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