Journal ArticleDOI
Minimum growth temperatures of Listeria monocytogenes and non‐haemolytic listeria
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TLDR
The results indicate that L. monocytogenes grows better than non-haemolytic strains under cold conditions, and the possible role of haemolysins as growth factors is discussed.Abstract:
Minimum growth temperatures and those of decreased growth were determined for 100 strains of listerias. The ability of 78 strains of Listeria monocytogenes isolated from animals and 22 non-haemolytic strains to grow at low temperatures was studied, using a flooding technique, in a plate-type continuous temperature gradient incubator at temperatures between -1.6 and 14.5 degrees C. The mean minimum temperature for L. monocytogenes was +1.7 +/- 0.5 degrees C. The growth of non-haemolytic listerias was unobservable at +1.7 +/- 0.5 degrees C. The L. monocytogenes strains grew at about 0.6 degrees C lower than the non-pathogenic strains. No differences in growth temperatures were observed among L. monocytogenes strains isolated from different sources. The serovars with the OI antigen grew at lower temperatures (+1.0 +/- 0.3 degrees C) than the other common serovar 4b (+1.3 +/- 0.4 degrees C). The results indicate that L. monocytogenes grows better than non-haemolytic strains under cold conditions. The possible role of haemolysins as growth factors is also discussed.read more
Citations
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Journal ArticleDOI
Listeria monocytogenes, a food-borne pathogen.
Jeffrey M. Farber,P I Peterkin +1 more
TL;DR: Improved methods for detecting and enumerating the organism in foodstuffs are now available, including those based on the use of monoclonal antibodies, DNA probes, or the polymerase chain reaction, which can help in the prevention and control of human infection.
Listeria monocytogenes, a food-borne pathogen
TL;DR: This article corrects the article on p. 485 in vol.
Journal ArticleDOI
Listeria monocytogenes: a multifaceted model
Melanie Hamon,Hélène Bierne,Hélène Bierne,Hélène Bierne,Pascale Cossart,Pascale Cossart,Pascale Cossart +6 more
TL;DR: The opportunistic intracellular pathogen Listeria monocytogenes has become a paradigm for the study of host–pathogen interactions and bacterial adaptation to mammalian hosts and the vast amount of knowledge gathered through in-depth comparative genomic analyses and in vivo studies makes it one of the most well-studied bacterial pathogens.
Journal ArticleDOI
A review of survival of pathogenic bacteria in organic waste used in biogas plants.
TL;DR: Temperature is the most important factor when considering the reduction of pathogens in BGP, but there are also other factors involved.
Journal ArticleDOI
Predictive modelling of the growth and survival of Listeria in fishery products
TL;DR: It is concluded that predictive models, successfully validated in agreement with defined performance criteria, will be an essential element of exposure assessment within formal quantitative risk assessment.
References
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Journal ArticleDOI
Epidemic Listeriosis — Evidence for Transmission by Food
W F Schlech rd,P M Lavigne,R A Bortolussi,A C Allen,E V Haldane,A J Wort,A.W. Hightower,S E Johnson,S H King,E S Nicholls,Claire V. Broome +10 more
TL;DR: The bacterium Listeria monocytogenes is a motile, gram-positive coccobacillus that can frequently be isolated from soil, water, and vegetation and is a common cause of meningoencephalitis and abort...
Journal ArticleDOI
Pasteurized milk as a vehicle of infection in an outbreak of listeriosis.
David W. Fleming,Stephen L. Cochi,Kristine L. MacDonald,Jack Brondum,Peggy S. Hayes,Brian D. Plikaytis,Marion B. Holmes,A. Audurier,Claire V. Broome,Arthur Reingold +9 more
TL;DR: The hypothesis that human listeriosis can be a foodborne disease is supported and questions about the ability of pasteurization to eradicate a large inoculum of L. monocytogenes from contaminated raw milk are raised.
Journal ArticleDOI
In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2.
TL;DR: Electron microscopic study demonstrated that bacteria from the nonhemolytic mutant remained inside phagosomes during cellular infection, whereas hemolytic bacteria from L. monocytogenes were released free within the cytoplasm, indicating that disruption of vacuole membranes by listeriolysin O-producing strains of L.monocytgenes might be a key mechanism allowing bacteria to escape from phagosome and to multiply unrestricted within cell cy toplasm.
Journal ArticleDOI
Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes.
TL;DR: It is strongly suggested that hemolysin is a major virulence factor implicated in the intracellular growth of L. monocytogenes, which is unable to grow in host tissues and were rapidly eliminated from the spleen and liver of infected mice.