Molecular characterization of a Leishmania donovanii cDNA clone with similarity to human 20S proteasome a-type subunit.
Claus Christensen,Louise Møller Jørgensen,Anja T. R. Jensen,S. Gasim,S. Gasim,Ming Chen,Arsalan Kharazmi,Thor G. Theander,K. Andresen +8 more
TLDR
In this paper, the authors isolated a Leishmania donovanii cDNA clone with similarity to the proteasome a-type subunit from humans and other eukaryotes.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 2000-01-03 and is currently open access. It has received 19 citations till now. The article focuses on the topics: cDNA library & Clone (cell biology).read more
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Nitric Oxide-Mediated Proteasome-Dependent Oligonucleosomal DNA Fragmentation in Leishmania amazonensis Amastigotes
Philippe Holzmuller,Denis Sereno,Mireille Cavaleyra,Isabelle Mangot,Sylvie Daulouède,Philippe Vincendeau,Jean-Loup Lemesre +6 more
TL;DR: No-induced oligonucleosomal DNA fragmentation in Leishmania amastigotes is, at least in part, regulated by noncaspase proteases of the proteasome, suggesting the determination of biochemical pathways leading up to cell death might ultimately allow the identification of new therapeutic targets.
Journal ArticleDOI
Characterization and role of protozoan parasite proteasomes
TL;DR: The present knowledge of proteasomes in protozoan parasites of medical importance such as Giardia, Entamoeba, Leishmania, Trypanosoma, Plasmodium and Toxoplasma spp.
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DNA Immunization with the Gene Encoding P4 Nuclease of Leishmania amazonensis Protects Mice against Cutaneous Leishmaniasis
TL;DR: The first report of successful use of a DNA vaccine to induce protection against L. amazonensis infection is reported, indicating that different vaccine combinations, including DNA encoding P4, HSP70, or IL-12, can provide significant protection against both Old World and New World cutaneous leishmaniasis.
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Antileishmanial activity of HIV protease inhibitors
TL;DR: New in vitro insights into the wide-spectrum efficacy of PI are added and it is suggested that studying their action on amastigote forms of leishmania within macrophages in order to validate their potential contribution against opportunistic infections in treated seropositive patients is suggested.
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Role of the Ubiquitin-Proteasome Systems in the Biology and Virulence of Protozoan Parasites.
TL;DR: Proteasomes are a key organelle in parasite biology and virulence and appear to be an attractive new chemotherapeutic target.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
TL;DR: The detection of murine leukemia virus antigens in complex cellular lysates was used to demonstrate the efficacy of this electrophoretic transfer technique.
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Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
TL;DR: By analyzing the effects of single base substitutions around the ATG initiator codon in a cloned preproinsulin gene, ACCATGG is identified as the optimal sequence for initiation by eukaryotic ribosomes.