Journal ArticleDOI
Molecular-mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny
TLDR
The structure of the genetic map is described and the existence of non-Mendelian segregations in linkage analysis as well as the origins of the observed distortions, are discussed.Abstract:
We have undertaken the construction of a Brassica napus genetic map with isozyme (4%), RFLP (26.5%) and RAPD (68%) markers on a 152 lines of a doubled-haploid population. The map covers 1765 cM and comprises 254 markers including three PCR-specific markers and a morphological marker. They are assembled into 19 linkage groups, covering approximatively 71% of the rapeseed genome. Thirty five percent of the studied markers did not segregate according to the expected Mendelian ratio and tended to cluster in eight specific linkage groups. In this paper, the structure of the genetic map is described and the existence of non-Mendelian segregations in linkage analysis as well as the origins of the observed distortions, are discussed. The mapped RFLP loci corresponded to the cDNAs already used to construct B. napus maps. The first results of intraspecific comparative mapping are presented.read more
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Journal ArticleDOI
Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome
Boulos Chalhoub,Shengyi Liu,Isobel A. P. Parkin,Haibao Tang,Haibao Tang,Xiyin Wang,Julien Chiquet,Harry Belcram,Chaobo Tong,Birgit Samans,Margot Correa,Corinne Da Silva,Jérémy Just,Cyril Falentin,Chu Shin Koh,Isabelle Le Clainche,Maria Bernard,Pascal Bento,Benjamin Noel,Karine Labadie,Adriana Alberti,Mathieu Charles,Dominique Arnaud,Hui Guo,Christian Daviaud,Salman Alamery,Kamel Jabbari,Kamel Jabbari,Meixia Zhao,Patrick P. Edger,Houda Chelaifa,David C. Tack,Gilles Lassalle,Imen Mestiri,Nicolas Schnel,Marie-Christine Le Paslier,Guangyi Fan,Victor Renault,Philippe E. Bayer,Agnieszka A. Golicz,Sahana Manoli,Tae-Ho Lee,Vinh Ha Dinh Thi,Smahane Chalabi,Qiong Hu,Chuchuan Fan,Reece Tollenaere,Yunhai Lu,Christophe Battail,Jinxiong Shen,Christine Sidebottom,Xinfa Wang,Aurélie Canaguier,Aurélie Chauveau,Aurélie Bérard,G. Deniot,Mei Guan,Zhongsong Liu,Fengming Sun,Yong Pyo Lim,Eric Lyons,Christopher D. Town,Ian Bancroft,Xiaowu Wang,Jinling Meng,Jianxin Ma,J. Chris Pires,Graham J.W. King,Dominique Brunel,Régine Delourme,Michel Renard,Jean-Marc Aury,Keith L. Adams,Jacqueline Batley,Jacqueline Batley,Rod J. Snowdon,Jörg Tost,David Edwards,David Edwards,Yongming Zhou,Wei Hua,Andrew G. Sharpe,Andrew H. Paterson,Chunyun Guan,Patrick Wincker,Patrick Wincker,Patrick Wincker +86 more
TL;DR: The polyploid genome of Brassica napus, which originated from a recent combination of two distinct genomes approximately 7500 years ago and gave rise to the crops of rape oilseed, is sequenced.
Journal ArticleDOI
Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers.
Joël Piquemal,E. Cinquin,F. Couton,C. Rondeau,E. Seignoret,I. doucet,D. Perret,M.-J. Villeger,Patrick Vincourt,Philippe Blanchard +9 more
TL;DR: A Brassica napus genetic map is constructed with 240 simple sequence repeats (SSR) primer pairs from private and public origins and could be used to identify more markers, which would eventually be linked to genes controlling important agronomic characters in rapeseed.
Journal ArticleDOI
A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content
Dan Qiu,Colin Morgan,Jiaqin Shi,Yan Long,Jun Liu,Ruiyan Li,X. Zhuang,Yan Wang,X. Tan,E. Dietrich,T. Weihmann,C. Everett,S. Vanstraelen,Paul Beckett,Fiona Fraser,Martin Trick,S. Barnes,J. Wilmer,Renate Schmidt,Jia Li,D. Li,J. Meng,Ian Bancroft +22 more
TL;DR: The results provide new insights into the genetic control of seed oil and erucic acid content in oilseed rape, and demonstrate the utility of the linkage map and population.
Journal ArticleDOI
DNA markers in plant improvement: An overview
TL;DR: The use of DNA marker technology has found application in fingerprinting genotypes, in determining seed purity, in systematic sampling of germplasm, and in phylogenetic analysis and this review discusses the use of this technology for the genetic improvement of plants.
Journal ArticleDOI
Genetic control of oil content in oilseed rape (Brassica napus L.)
Régine Delourme,C. Falentin,Virginie Huteau,V. Clouet,R. Horvais,B. Gandon,S. Specel,L. Hanneton,J. E. Dheu,M. Deschamps,E. Margale,P. Vincourt,Maurice Renard +12 more
TL;DR: The results show the possibility to combine favourable alleles at different QTL to increase seed oil content and to use Arabidopsis genomic data to derive markers for oilseed rape QTL and identify candidate genes, as well as the interest to combine information from different segregating populations in order to build a consolidated map of QTL involved in a specific trait.
References
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Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations
Eric S. Lander,Eric S. Lander,Philip Green,Jeff Abrahamson,Aaron Barlow,Mark J. Daly,Stephen E Lincoln,Lee Newburg +7 more
TL;DR: A computer package, called MAPMAKER, designed specifically for the construction of linkage maps in a number of organisms, including the human and several plants, and it is outlined the mapping strategies that have been used.
Journal ArticleDOI
The estimation of map distances from recombination values.
TL;DR: The genetic map is a tool to quantify the distance between genes on a chromosome, based on the observed frequency of crossovers during cell division, which is used to estimate the total distance between chromosomes.
Journal ArticleDOI
Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.
TL;DR: Bulk segregant analysis has several advantages over the use of near-isogenic lines to identify markers in specific regions of the genome and will have widespread application both in those species where selfing is possible and in those that are obligatorily outbreeding.