Nano-topography sensing by osteoclasts.
TLDR
It was observed that steps or sub-micrometer cracks on the smooth surface stimulate local ring formation, raising the possibility that similar imperfections on bone surfaces may stimulate local osteoclast resorptive activity.Abstract:
Bone resorption by osteoclasts depends on the assembly of a specialized, actin-rich adhesive ‘sealing zone’ that delimits the area designed for degradation. In this study, we show that the level of roughness of the underlying adhesive surface has a profound effect on the formation and stability of the sealing zone and the associated F-actin. As our primary model substrate, we use ‘smooth’ and ‘rough’ calcite crystals with average topography values of 12 nm and 530 nm, respectively. We show that the smooth surfaces induce the formation of small and unstable actin rings with a typical lifespan of ~8 minutes, whereas the sealing zones formed on the rough calcite surfaces are considerably larger, and remain stable for more than 6 hours. It was further observed that steps or sub-micrometer cracks on the smooth surface stimulate local ring formation, raising the possibility that similar imperfections on bone surfaces may stimulate local osteoclast resorptive activity. The mechanisms whereby the physical properties of the substrate influence osteoclast behavior and their involvement in osteoclast function are discussed.read more
Citations
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Journal ArticleDOI
Topography-mediated immunomodulation in osseointegration; Ally or Enemy.
TL;DR: In this paper , a review summarizes current information regarding immune cell-titanium implant surface interactions and places these events in the context of surface mediated immunomodulation and bone regeneration.
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L-Plastin deficiency produces increased trabecular bone due to attenuation of sealing ring formation and osteoclast dysfunction.
Meenakshi A. Chellaiah,Megan C. Moorer,Sunipa Majumdar,Hanan Aljohani,Sharon Celeste Morley,Vanessa R. Yingling,Joseph P. Stains +6 more
TL;DR: It is shown that L-plastin knockout mice produce osteoclasts that are deficient in the formation of NSZs, are hyporesorptive, and make superficial resorption pits in vitro, and could be a potential target for therapeutic interventions to treat trabecular bone loss.
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Cell Adhesion to the Extracellular Matrix
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TL;DR: The main characteristics of integrin adhesions are reviewed, in intact animals and in cultured cells, addressing their complex nano-architecture, molecular heterogeneity, and dynamic reorganization.
BookDOI
Stem Cells and Cancer Stem Cells, Volume 7
TL;DR: The present report has introduced a novel outlook for hematopoietic stem cells (HSCs) therapy on the basis of structural and functional interpretation of stem cell factor receptor (c-Kit), which can change the perspectives of oncogene-oriented structural biology approach.
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Drug delivery and cell interaction of adhesive poly(ethyleneimine)/sulfated polysaccharide complex particle films
Martin Müller,Bernhard Torger,Diana Wehrum,David Vehlow,Birgit Urban,Beatrice Woltmann,Ute Hempel +6 more
TL;DR: ZOL-loaded PEI/CS nanoparticles, which were immobilized on an osteoblast-derived extracellular matrix, reduced significantly the resorption and the metabolic activity of human monocyte-derived osteoclasts.
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