Network analysis of intermediary metabolism using linear optimization. I. Development of mathematical formalism.
TLDR
Analysis of metabolic networks using linear optimization theory allows one to quantify and understand the limitations imposed on the cell by its metabolic stoichiometry, and to understand how the flux through each pathway influences the overall behavior of metabolism.About:
This article is published in Journal of Theoretical Biology.The article was published on 1992-02-21 and is currently open access. It has received 255 citations till now.read more
Citations
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Journal ArticleDOI
Predictions for Oxygen Supply Control to Enhance Population Stability of Engineered Production Strains
TL;DR: It is demonstrated that although an appropriate choice of oxygenation may increase culture longevity more than twofold, total production as governed by economic criterion can be increased by several orders of magnitude.
Journal ArticleDOI
Design principles and operating principles: the yin and yang of optimal functioning.
TL;DR: It is shown that flux split ratios are essentially sufficient for unregulated, but not for regulated branch points, and the question arises whether the consideration of non-linearities is really necessary for devising optimal strategies for yield improvements.
Journal ArticleDOI
Insights into the Central Metabolism of Spodoptera frugiperda (Sf‐9) and Trichoplusia ni BTI‐Tn‐5B1‐4(Tn‐5) Insect Cells by Radiolabeling Studies
TL;DR: This work has analyzed the metabolism of glucose and glutamine, the main carbon and/or energy compounds, of the two most commonly used insect cell lines, Spodoptera frugiperda (Sf‐9) and the Trichoplusia niBTI‐Tn‐5B1‐4 (Tn-5).
Journal ArticleDOI
An in silico re-design of the metabolism in Thermotoga maritima for increased biohydrogen production
TL;DR: An updated genome-scale metabolic model of T. maritima was used to describe in detail the H2 metabolism in this bacterium, identify suitable carbon sources for enhancing H2 production, and design knockout strains, which increased the in silico hydrogen production up to 20%.
Book ChapterDOI
Metabolic networks and their evolution.
TL;DR: Two such changes are discussed, namely the elimination of reactions from a metabolic network through loss of function mutations in enzyme-coding genes, and the addition of metabolic reactions, for example through mechanisms such as horizontal gene transfer.
References
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Book
Linear and nonlinear programming
David G. Luenberger,Yinyu Ye +1 more
TL;DR: Strodiot and Zentralblatt as discussed by the authors introduced the concept of unconstrained optimization, which is a generalization of linear programming, and showed that it is possible to obtain convergence properties for both standard and accelerated steepest descent methods.
Journal ArticleDOI
The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. Role of mitochondrial NAD(P)+-dependent malic enzyme.
R W Moreadith,A L Lehninger +1 more
TL;DR: Analysis of oxidative pathways of glutamine and glutamate showed that extramitochondrial malate is oxidized almost quantitatively to pyruvate + CO2 by NAD(P)+-linked malic enzyme, present in the mitochondria of all tumors tested, but absent in heart, liver, and kidney mitochondria.