New dithiolopyrrolone antibiotics induced by adding sorbic acid to the culture medium of Saccharothrix algeriensis NRRL B-24137.
Rabiâa Merrouche,Noureddine Bouras,Yannick Coppel,Yannick Coppel,Florence Mathieu,Nasserdine Sabaou,Ahmed Lebrihi +6 more
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Four new antibiotics were purified from the fermentation broth of Saccharothrix algeriensis NRRL B-24137 and characterized as dithiolopyrrolone derivatives, which were induced by adding sorbic acid, as precursor, at a concentration of 5 mM to the semi-synthetic medium.Abstract:
Dithiolopyrrolone antibiotics, produced by several microorganisms, are known for their strong antimicrobial activities. This class of antibiotics generated new interest after the discovery of their anticancer and antitumor properties. In this study, four new antibiotics were purified from the fermentation broth of Saccharothrix algeriensis NRRL B-24137 and characterized as dithiolopyrrolone derivatives. These new dithiolopyrrolone antibiotics were induced by adding sorbic acid, as precursor, at a concentration of 5 mM to the semi-synthetic medium. The analysis of the induced antibiotics was carried out by HPLC. The maximal production of the antibiotics PR2, PR8, PR9 and PR10 was 0.08±0.04, 0.21±0.04, 0.13±0.03 and 0.09±0.00 mg L(-1) , respectively, obtained after 8 days of fermentation. The chemical structures of these antibiotics were determined by (1) H- and (13) C-nuclear magnetic resonance, mass and UV-visible data. The four new dithiolopyrrolone antibiotics - PR2, PR8, PR9 and PR10 - were characterized, respectively, as crotonyl-pyrrothine, sorbyl-pyrrothine, 2-hexonyl-pyrrothine and 2-methyl-3-pentenyl-pyrrothine. The minimum inhibitory concentrations of the new induced antibiotics were determined.read more
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Dithiolopyrrolones: biosynthesis, synthesis, and activity of a unique class of disulfide-containing antibiotics.
TL;DR: This review will comprehensively cover the history and development of dithiolopyrrolones with particular emphasis on the biosynthesis, synthesis, biological activity and mechanism of action.
Journal ArticleDOI
Dithiolopyrrolone natural products: isolation, synthesis and biosynthesis.
TL;DR: The aim of this review is to give an overview about the isolations, characterizations, synthesis, biosynthesis, bioactivities and mode of action of this unique family of dithiolopyrrolone natural products, focusing on the period from 1940s until now.
Journal ArticleDOI
Taxonomy and chemical characterization of new antibiotics produced by Saccharothrix SA198 isolated from a Saharan soil.
Dalila Boubetra,Nasserdine Sabaou,Abdelghani Zitouni,Christian Bijani,Christian Bijani,Ahmed Lebrihi,Florence Mathieu +6 more
TL;DR: Actinomycete strain SA198, isolated from a Saharan soil sample of Algeria, exhibited antimicrobial activity against Gram-positive and Gram-negative bacteria, and phytopathogenic and toxinogenic fungi, consistent with those of the genus Saccharothrix.
Journal ArticleDOI
Efficiency of Saccharothrix algeriensis NRRL B-24137 and Its Produced Antifungal Dithiolopyrrolones Compounds to Suppress Fusarium oxysporum-Induced Wilt Disease Occurring in Some Cultivated Crops
Rabiâa Merrouche,Amine Yekkour,Lynda Lamari,Abdelghani Zitouni,Florence Mathieu,Nasserdine Sabaou +5 more
TL;DR: This screening showed the significant antifusarium activity of the actinobacteria strain against FOLi, FOC, FOLe and FOLy, which suggested the involvement of more than direct antibiosis in the whole SA biocontrol performance.
Journal ArticleDOI
Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules.
TL;DR: Focusing on the Algerian natural sources of antimicrobial metabolites, this work is a representative example of the potential of a closely combined study on biology and chemistry of natural products.
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