NITROGEN LIMITATION ADAPTATION, a Target of MicroRNA827, Mediates Degradation of Plasma Membrane–Localized Phosphate Transporters to Maintain Phosphate Homeostasis in Arabidopsis
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TLDR
Posttranslational regulation of Pi transport is revealed through modulation of degradation of PHT1 proteins by the RING-type ubiquitin E3 ligase, NITROGEN LIMITATION ADAPTATION (NLA).Abstract:
Members of the Arabidopsis thaliana PHOSPHATE TRANSPORTER1 (PHT1) family are key players in acquisition of Pi from the rhizosphere, and their regulation is indispensable for the maintenance of cellular Pi homeostasis. Here, we reveal posttranslational regulation of Pi transport through modulation of degradation of PHT1 proteins by the RING-type ubiquitin E3 ligase, NITROGEN LIMITATION ADAPTATION (NLA). Loss of function of NLA caused high Pi accumulation resulting from increases in the levels of several PHT1s at the protein rather than the transcript level. Evidence of decreased endocytosis and ubiquitination of PHT1s in nla mutants and interaction between NLA and PHT1s in the plasma membranes suggests that NLA directs the ubiquitination of plasma membrane–localized PHT1s, which triggers clathrin-dependent endocytosis followed by endosomal sorting to vacuoles. Furthermore, different subcellular localization of NLA and PHOSPHATE2 (PHO2; a ubiquitin E2 conjugase) and the synergistic effect of the accumulation of PHT1s and Pi in nla pho2 mutants suggest that they function independently but cooperatively to regulate PHT1 protein amounts. Intriguingly, NLA and PHO2 are the targets of two Pi starvation-induced microRNAs, miR827 and miR399, respectively. Therefore, our findings uncover modulation of Pi transport activity in response to Pi availability through the integration of a microRNA-mediated posttranscriptional pathway and a ubiquitin-mediated posttranslational regulatory pathway.read more
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Root microbiota drive direct integration of phosphate stress and immunity
Gabriel Castrillo,Paulo José Pereira Lima Teixeira,Sur Herrera Paredes,Theresa F. Law,Laura de Lorenzo,Meghan E. Feltcher,Omri M. Finkel,Natalie W. Breakfield,Natalie W. Breakfield,Piotr A. Mieczkowski,Corbin D. Jones,Javier Paz-Ares,Jeffery L. Dangl +12 more
TL;DR: It is established that a genetic network controlling the phosphate stress response influences the structure of the root microbiome community, even under non-stress phosphate conditions, and that the master transcriptional regulators of phosphate Stress Response in Arabidopsis thaliana directly repress defence, consistent with plant prioritization of nutritional stress over defence.
Journal ArticleDOI
SPX1 is a phosphate-dependent inhibitor of PHOSPHATE STARVATION RESPONSE 1 in Arabidopsis
María Isabel Puga,I. Mateos,Rajulu Charukesi,Zhiye Wang,José Manuel Franco-Zorrilla,Laura de Lorenzo,M. L. Irigoyen,Simona Masiero,Regla Bustos,Jose Rodriguez,Antonio Leyva,Vicente Rubio,Hans Sommer,Javier Paz-Ares +13 more
TL;DR: DNA-binding and pull-down assays showed that SPX1 is a competitive inhibitor of PHR1 binding to its recognition sequence, and that its efficiency is highly dependent on the presence of Pi or phosphite, a nonmetabolizable Pi analog that can repress PSRs.
Journal ArticleDOI
Molecular mechanisms underlying phosphate sensing, signaling, and adaptation in plants.
TL;DR: In this review, recent advances are evaluated in terms of progress toward developing a comprehensive understanding of the molecular events underlying control over P homeostasis in crop plants from the perspective of agricultural sustainability in the face of diminishing global P supplies.
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Stress induced gene expression drives transient DNA methylation changes at adjacent repetitive elements
David Secco,Chuang Wang,Huixia Shou,Matthew D. Schultz,Serge Chiarenza,Laurent Nussaume,Joseph R. Ecker,James Whelan,Ryan Lister +8 more
TL;DR: In this paper, the authors used whole genome bisulfite sequencing to assess the spatio-temporal dynamics of mC in rice grown under phosphate starvation and recovery conditions, and identified widespread phosphate starvation-induced changes in mC, preferentially localized in transposable elements (TEs) close to highly induced genes.
Journal ArticleDOI
NITROGEN LIMITATION ADAPTATION Recruits PHOSPHATE2 to Target the Phosphate Transporter PT2 for Degradation during the Regulation of Arabidopsis Phosphate Homeostasis
TL;DR: The results suggest that this downregulation relieves the posttranslational repression of PT2, allowing it to accumulate and participate in Pi uptake, and provides additional molecular details describing Pi signaling/homeostasis regulation by identifying NLA and UBC24 as partners and PT2 as one of their downstream targets.
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