Nod factors potentiate auxin signaling for transcriptional regulation and lateral root formation in Medicago truncatula.
Violaine Herrbach,Ximena Chirinos,David Rengel,Kokoévi Agbevenou,Rémy Vincent,Stéphanie Pateyron,Stéphanie Pateyron,Stéphanie Huguet,Stéphanie Huguet,Sandrine Balzergue,Sandrine Balzergue,Asher Pasha,Nicholas J. Provart,Clare Gough,Sandra Bensmihen +14 more
TLDR
Using both pharmacological and transcriptomic approaches, it is shown that Nod factors and auxin interact synergistically to control lateral root formation and gene regulatory responses.Abstract:
Nodulation (Nod) factors (NFs) are symbiotic molecules produced by rhizobia that are essential for establishment of the rhizobium-legume endosymbiosis. Purified NFs can stimulate lateral root formation (LRF) in Medicago truncatula, but little is known about the molecular mechanisms involved. Using a combination of reporter constructs, pharmacological and genetic approaches, we show that NFs act on early steps of LRF in M. truncatula, independently of the ethylene signaling pathway and of the cytokinin receptor MtCRE1, but in interaction with auxin. We conducted a whole-genome transcriptomic study upon NF and/or auxin treatments, using a lateral root inducible system adapted for M. truncatula. This revealed a large overlap between NF and auxin signaling and, more interestingly, synergistic interactions between these molecules. Three groups showing interaction effects were defined: group 1 contained more than 1500 genes responding specifically to the combinatorial treatment of NFs and auxin; group 2 comprised auxin-regulated genes whose expression was enhanced or antagonized by NFs; and in group 3 the expression of NF regulated genes was antagonized by auxin. Groups 1 and 2 were enriched in signaling and metabolic functions, which highlights important crosstalk between NF and auxin signaling for both developmental and symbiotic processes.read more
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Nodule inception recruits the lateral root developmental program for symbiotic nodule organogenesis in Medicago truncatula
Katharina Schiessl,Katharina Schiessl,Jodi L. Stewart Lilley,Tak Lee,Ioannis Tamvakis,Ioannis Tamvakis,Wouter Kohlen,Paul Bailey,Aaron Thomas,Jakub Luptak,Karunakaran Ramakrishnan,Matthew D. Carpenter,Kirankumar S. Mysore,Jiangqi Wen,Sebastian E. Ahnert,Verônica A. Grieneisen,Giles E. D. Oldroyd,Giles E. D. Oldroyd +17 more
TL;DR: It is demonstrated that despite differential induction, lateral roots and nodules share overlapping developmental programs, with mutants in LOB-DOMAIN PROTEIN 16 (LBD16) showing equivalent defects in nodule and lateral root initiation.
Journal ArticleDOI
LysM Receptor-Like Kinase and LysM Receptor-Like Protein Families: An Update on Phylogeny and Functional Characterization.
TL;DR: A phylogeny of these families in eight plant species, including dicotYledons and monocotyledons, is provided, and known or putative biological roles of the members in each of the identified phylogenetic groups are discussed.
Journal ArticleDOI
Auxin transport, metabolism, and signalling during nodule initiation: indeterminate and determinate nodules.
TL;DR: This work reviews how auxin contributes to early nodule development and focuses on changes in auxin transport, signalling, and metabolism during nodule initiation, describing both experimental evidence and computer modelling.
Journal ArticleDOI
Mini-Review: Nod Factor Regulation of Phytohormone Signaling and Homeostasis During Rhizobia-Legume Symbiosis.
TL;DR: This review will focus on the emerging notion of NF and phytohormone signaling crosstalk, and how it could contribute to the tight control of symbiosis establishment in legume host plants.
Journal ArticleDOI
MtLAX2, a Functional Homologue of the Arabidopsis Auxin Influx Transporter AUX1, Is Required for Nodule Organogenesis
Sonali Roy,Fran Robson,Jodi L. Stewart Lilley,Cheng-Wu Liu,Xiaofei Cheng,Jiangqi Wen,Simon Walker,Jongho Sun,Donna Cousins,Caitlin Bone,Malcolm J. Bennett,J. Allan Downie,Ranjan Swarup,Giles E. D. Oldroyd,Jeremy D. Murray +14 more
TL;DR: The data indicate that MtLAX2 is a functional analog of Arabidopsis AUX1 and is required for the accumulation of auxin during nodule formation in tissues underlying sites of rhizobial infection.
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