Journal ArticleDOI
Nuclear receptor that identifies a novel retinoic acid response pathway
TLDR
By indicating the existence of an additional pathway through which retinoic acid may exert its effects, these data lead to a re-evaluation of retinoid physiology.Abstract:
Molecular cloning and transcriptional activation studies have revealed a new protein similar to the steroid hormone receptors and which responds specifically to vitamin A metabolites. This protein is substantially different in primary structure and ligand specificity from the products of the previously described retinoic acid receptor gene family. By indicating the existence of an additional pathway through which retinoic acid may exert its effects, these data lead to a re-evaluation of retinoid physiology.read more
Citations
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The nuclear receptor superfamily: the second decade.
David J. Mangelsdorf,Carl S. Thummel,Miguel Beato,Peter Herrlich,Günther Schütz,Kazuhiko Umesono,Bruce Blumberg,Philippe Kastner,Manuel Mark,Pierre Chambon,Ronald M. Evans +10 more
TL;DR: This research presents a new probabilistic procedure called ‘spot-spot analysis’ to characterize the response of the immune system to the presence of E.coli.
Journal ArticleDOI
Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor.
TL;DR: The results suggest that the physiologic role of PPAR gamma 2 is to regulate development of the adipose lineage in response to endogenous lipid activators and that this factor may serve to link the process of adipocyte differentiation to systemic lipid metabolism.
Journal ArticleDOI
The RXR heterodimers and orphan receptors
TL;DR: The historical links between the steroid and nonsteroid receptor signaling systems are established, the explosive development of the retinoid X receptor (RXR) heterodimer and orphan receptor family is charted, the impact of these discoveries on the authors' understanding of the mechanisms of hormonal signaling is explained, and emerging issues and implications are presented.
Journal ArticleDOI
mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer.
TL;DR: In this paper, an enhancer from the 5'-flanking region of the adipocyte P2 (aP2) gene that directs high-level adipocyte-specific gene expression in both cultured cells and transgenic mice was identified.
PatentDOI
Transcriptional co-repressor that interacts with nuclear hormone receptors
Ronald M. Evans,J. Don Chen +1 more
TL;DR: A receptor-interacting factor, SMRT, is identified as a silencing mediator (co-repressor) for retinoid and thyroid-hormone receptors and a new class of cofactors which may be important mediators of hormone action are identified.
References
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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A comprehensive set of sequence analysis programs for the VAX
TL;DR: A group of programs that will interact with each other has been developed for the Digital Equipment Corporation VAX computer using the VMS operating system.
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The steroid and thyroid hormone receptor superfamily
TL;DR: A superfamily of regulatory proteins that include receptors for thyroid hormone and the vertebrate morphogen retinoic acid is identified, suggesting mechanisms underlying morphogenesis and homeostasis may be more ubiquitous than previously expected.
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
TL;DR: A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.