Patterns of Amino Acids near Signal‐Sequence Cleavage Sites
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TLDR
In this paper, some such patterns, based on a sample of 78 eukaryotic signal sequences, are presented and discussed, and a first attempt at formulating rules for the prediction of cleavage sites is made.Abstract:
According to the signal hypothesis, a signal sequence, once having initiated export of a growing protein chain across the rough endoplasmic reticulum, is cleaved from the mature protein at a specific site. It has long been known that some part of the cleavage specificity resides in the last residue of the signal sequence, which invariably is one with a small, uncharged side-chain, but no further specific patterns of amino acids near the point of cleavage have been discovered so far. In this paper, some such patterns, based on a sample of 78 eukaryotic signal sequences, are presented and discussed, and a first attempt at formulating rules for the prediction of cleavage sites is made.read more
Citations
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Journal ArticleDOI
A new method for predicting signal sequence cleavage sites.
TL;DR: A new method for identifying secretory signal sequences and for predicting the site of cleavage between a signal sequence and the mature exported protein is described.
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Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.
TL;DR: A neural network-based tool, TargetP, for large-scale subcellular location prediction of newly identified proteins has been developed and it is estimated that 10% of all plant proteins are mitochondrial and 14% chloroplastic, and that the abundance of secretory proteins, in both Arabidopsis and Homo, is around 10%.
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A combined transmembrane topology and signal peptide prediction method.
TL;DR: Phobius, a combined transmembrane protein topology and signal peptide predictor based on a hidden Markov model, noted a drastic reduction of false classifications compared to TMHMM/SignalP, suggesting that Phobius is well suited for whole-genome annotation of signal peptides and trans Membrane regions.
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Molecular cloning and expression of human hepatocyte growth factor
Toshikazu Nakamura,Tsutomu Nishizawa,Mitchio Hagiya,Tatsuya Seki,Manabu Shimonishi,Atsushi Sugimura,Kosuke Tashiro,Shin Shimizu +7 more
TL;DR: The nucleotide sequence of the human HGF cDNA reveals that both α- andβ-chains are contained in a single open reading frame coding for a pre-pro precursor protein of 728 amino acids, which indicates that the activity of HGF is not species-specific.
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Signal sequences: The limits of variation
TL;DR: The results show subtle differences between eukaryotic and prokaryotic sequences, but the general impression of signal sequences as being highly variable is reinforced.
References
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Hitoshi Kakidani,Yasuji Furutani,Hideo Takahashi,Masaharu Noda,Yuuki Morimoto,Tadaaki Hirose,Michiko Asai,Seiichi Inayama,Shigetada Nakanishi,Shosaku Numa +9 more
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