Journal ArticleDOI
OsPPR1, a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis.
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TLDR
The results suggest that the OsPPR1 is a nuclear gene of rice, encoding the PPR protein that might play a role in the chloroplast biogenesis.Abstract:
In this paper, we report a novel pentatricopeptide repeat (PPR) protein gene in rice. PPR, a characteristic repeat motif consisted of tandem 35 amino acids, has been found in various biological systems including plant. Sequence analysis revealed that the gene designated OsPPR1 consisted of an open reading frame of 2433 nucleotides encoding 810 amino acids that include 11 PPR motifs. Blast search result indicated that the gene did not align with any of the characterized PPR genes in plant. The OsPPR1 gene was found to contain a putative chloroplast transit peptide in the N-terminal region, suggesting that the gene product targets to the chloroplast. Southern blot hybridization indicated that the OsPPR1 is the member of a gene family within the rice genome. Expression analysis and immunoblot analysis suggested that the OsPPR1 was accumulated mainly in rice leaf. Antisense transgenic strategy was used to suppress the expression of OsPPR1 and the resulted transgenic rice showed the typical phenotypes of chlorophyll-deficient mutants; albinism and lethality. Cytological observation using microscopy revealed that the antisense transgenic plant contained a significant defect in the chloroplast development. Taken together, the results suggest that the OsPPR1 is a nuclear gene of rice, encoding the PPR protein that might play a role in the chloroplast biogenesis. This is the first report on the PPR protein required for the chloroplast biogenesis in rice.read more
Citations
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Pentatricopeptide repeat proteins: a socket set for organelle gene expression.
TL;DR: Several recent papers are discussed that cover the evolutionary history and molecular mode of action of Pentatricopeptide repeat proteins, and propose hypotheses for their physiological roles that could explain why PPR proteins are so numerous in terrestrial plants.
Journal ArticleDOI
Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing
Zhonghua Wang,Yanjiao Zou,Xiaoyu Li,Qunyu Zhang,Letian Chen,Hao Wu,Dihua Su,Yuanling Chen,Jingxin Guo,Da Luo,Da Luo,Yunming Long,Yang Zhong,Yao-Guang Liu +13 more
TL;DR: It is shown in rice (Oryza sativa) with Boro II cytoplasm that an abnormal mitochondrial open reading frame, orf79, is cotranscribed with a duplicated atp6 (B-atp6) gene and encodes a cytotoxic peptide and plays an additional role in promoting the editing of atp 6 mRNAs, independent of its cleavage function.
Journal ArticleDOI
Stress‐responsive microRNAs in Populus
TL;DR: The cloning of small RNAs from abiotic stressed tissues of Populus trichocarpa (Ptc) and the identification of 68 putative miRNA sequences that can be classified into 27 families based on sequence homology are reported, which suggests that the members of a family may have different functions.
Journal ArticleDOI
CLB19, a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts.
Anne-Laure Chateigner-Boutin,Maricela Ramos-Vega,Arturo Guevara-García,Charles Andrés,Charles Andrés,María de la Luz Gutiérrez-Nava,Araceli Cantero,Etienne Delannoy,Luis F. Jiménez,Claire Lurin,Ian Small,Ian Small,Patricia León +12 more
TL;DR: In this paper, the authors characterized CLB19 (also known as PDE247), a gene that is required for editing two distinct chloroplast transcripts, rpoA and clpP.
Journal ArticleDOI
RNA editing in plant organelles: machinery, physiological function and evolution
TL;DR: The possible steps of co-evolution of RNA editing events and PPR proteins are discussed.
References
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