Journal ArticleDOI
Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application.
Eric Record,Michèle Asther,C. Sigoillot,Sandrine Pagès,Sandrine Pagès,Peter J. Punt,Michel Delattre,Mireille Haon,C.A.M.J.J. van den Hondel,Jean-Claude Sigoillot,Laurence Lesage-Meessen,Marcel Asther +11 more
TLDR
The recombinant FAEA was tested for wheat straw pulp bleaching, with or without a laccase mediator system and xylanase, and yielded very efficient delignification—close to 75%—and a kappa number of 3.9.Abstract:
A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l-1), improved FAEA activity 24.5-fold and a yield of 1 g l-1 of the corresponding protein in the culture medium was achieved. The secreted FAEA was purified 3.5-fold to homogeneity in a two-step purification procedure with a recovery of 69%. The overproduced protein was characterised and presented properties in good agreement with those of native FAEA. The recombinant FAEA was tested for wheat straw pulp bleaching, with or without a laccase mediator system and xylanase. Best results were obtained using a bi-sequential process with a sequence including xylanase, FAEA and laccase, and yielded very efficient delignification - close to 75% - and a kappa number of 3.9. This is the first report on the potential application of recombinant FAEA in the pulp and paper sector. Chemicals/CAS: hemicellulose, 63100-39-0, 63100-40-3, 9034-32-6; laccase, 80498-15-3; xylan endo 1,3 beta xylosidase, 37278-89-0, 9025-55-2; Carboxylic Ester Hydrolases, EC 3.1.1.-; Endo-1,4-beta Xylanases, EC 3.2.1.8; feruloyl esterase, EC 3.1.1.73; Laccase, EC 1.10.3.2; Recombinant Proteinsread more
Citations
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Journal ArticleDOI
Enzymatic saccharification of wheat straw for bioethanol production by a combined cellulase xylanase and feruloyl esterase treatment
TL;DR: Optimisation of enzymatic hydrolyses allowed the use of lower quantities of enzymes and improved the cost effectiveness of the process.
Journal ArticleDOI
Developing Aspergillus as a host for heterologous expression
TL;DR: History of the development of Aspergillus as an expression host, current state of the art and future directions are reviewed, touching on related research in other fungi when discussing the areas of greatest potential for future biotechnological applications.
Journal ArticleDOI
Microbial production, characterization and applications of feruloyl esterases
TL;DR: FAEs act synergistically with xylanases to hydrolyze ester-linked ferulic and diferulic acid from cell wall material and therefore play a major role in the degradation of plant biomass.
Journal ArticleDOI
Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis
Dyoni Matias de Oliveira,Aline Finger-Teixeira,Thatiane R. Mota,Victor Hugo Salvador,Flávia Carolina Moreira-Vilar,Hugo Bruno Correa Molinari,Rowan A. C. Mitchell,Rogério Marchiosi,Osvaldo Ferrarese-Filho,Wanderley Dantas dos Santos +9 more
TL;DR: The biosynthesis and roles of FA in cell wall architecture and in grass biomass recalcitrance to enzyme hydrolysis are reviewed.
Journal ArticleDOI
Biotechnological applications and potential of fungal feruloyl esterases based on prevalence, classification and biochemical diversity
TL;DR: This review provides an overview of the current knowledge on fungal feruloyl esterases focussing in particular on the differences in substrate specificity, regulation of their production, prevalence of these enzymes in fungal genomes and industrial applications.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
Laccase: new functions for an old enzyme.
TL;DR: This work has focused on more recent reports on the occurrence of laccase and its functions in physiological development and industrial utility and the reports of molecular weights, pH optima, and substrate specificity are extremely diverse.
Journal ArticleDOI
History, overview and applications of mediated lignolytic systems, especially laccase-mediator-systems (Lignozym-process)
H.P. Call,I. Mücke +1 more
TL;DR: The general applicability of the laccase-mediator-system (Lignozym®-process) will be given by a comparison of results obtained with pulps from different sources of wood and from several pulping processes, especially regarding effects on pulps, kinetic data and specificity of the reaction.
Journal ArticleDOI
Application of enzymes in the pulp and paper industry
TL;DR: A wide range of applications in the pulp and paper industry have now been identified, and the most important application of enzymes is in the prebleaching of kraft pulp, where Xylanase enzymes have been found to be most effective.
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