Journal ArticleDOI
Parallel expression profiling of barley - Stem rust interactions
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This analysis provides a list of candidate signaling components, which can be analyzed for function in Rpg1-mediated disease resistance, which were activated during the early infection process, before the hypersensitive response or fungal growth inhibition occurred.Abstract:
The dominant barley stem rust resistance gene Rpg1 confers resistance to many but not all pathotypes of the stem rust fungus Puccinia graminis f. sp. tritici (Pgt). Transformation of Rpg1 into susceptible cultivar Golden Promise rendered the transgenic plants resistant to Pgt pathotype MCC but not to Pgt pathotype QCC. Our objective was to identify genes that are induced/repressed during the early stages of pathogen infection to elucidate the molecular mechanisms and role of Rpg1 in defense. A messenger ribonucleic acid expression analysis using the 22K Barley1 GeneChip was conducted in all pair-wise combinations of two isolines (cv. Golden Promise and Rpg1 transgenic line G02-448F-3R) and two Pgt pathotypes (MCC and QCC) across six time points. Analysis showed that a total of 34 probe sets exhibited expression pattern differences between Golden Promise (susceptible) and G02-448F-3R (resistant) infected with Pgt-MCC. A total of 14 probe sets exhibited expression pattern differences between Pgt-MCC (avirulent) and Pgt-QCC (virulent) inoculated onto G02-448F-3R. These differentially expressed genes were activated during the early infection process, before the hypersensitive response or fungal growth inhibition occurred. Our analysis provides a list of candidate signaling components, which can be analyzed for function in Rpg1-mediated disease resistance.read more
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Journal ArticleDOI
Plant glutathione transferase-mediated stress tolerance: functions and biotechnological applications
Irini Nianiou-Obeidat,Panagiotis Madesis,Christos Kissoudis,Christos Kissoudis,Georgia Voulgari,Evangelia G. Chronopoulou,Athanasios Tsaftaris,Nikolaos E. Labrou +7 more
TL;DR: The biotechnological applications of GSTs towards developing plants that are resistant to biotic and abiotic stresses are reviewed and it is proposed that future GST engineering efforts through rational and combinatorial approaches, would lead to the design of improved isoenzymes with purpose-designed catalytic activities and novel functional properties.
Journal ArticleDOI
The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains
Robert Brueggeman,Arnis Druka,Jayaveeramuthu Nirmala,T. Cavileer,Tom Drader,Nils Rostoks,Aghafakhr Mirlohi,H. Bennypaul,Upinder S. Gill,David Kudrna,C. Whitelaw,A. Kilian,F. Han,Y. Sun,Kulvinder S. Gill,Brian J. Steffenson,Andris Kleinhofs +16 more
TL;DR: Allele and recombinant sequencing suggested that the probable rpg4 gene encoded an actin depolymerizing factor-like protein, and allele sequencing validated the candidate Rpg5 gene, which is expressed at low but detectable levels.
Journal ArticleDOI
An eQTL analysis of partial resistance to Puccinia hordei in barley.
Xinwei Chen,Christine A. Hackett,Rients E. Niks,Pete E. Hedley,Clare Booth,Arnis Druka,Thierry C. Marcel,Anton Vels,Micha Bayer,Iain Milne,Jenny Morris,Luke Ramsay,David Marshall,Linda Cardle,Robbie Waugh +14 more
TL;DR: The eQTL approach yielded information that led to the identification of strong candidate genes underlying pQTL for resistance to leaf rust in barley and on the general pathogen response pathway, and will facilitate a systems appraisal of this host-pathogen interaction and, potentially, for other traits measured in this population.
Journal ArticleDOI
Blufensin1 Negatively Impacts Basal Defense in Response to Barley Powdery Mildew
TL;DR: Barley stripe mosaic virus-induced gene silencing of Bln1 enhanced plant resistance in compatible interactions, regardless of the presence or absence of functional Mla coiled-coil, nucleotide-binding site, Leu-rich repeat alleles, indicating that BLN1 can function in an R-gene-independent manner.
Journal ArticleDOI
Quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons
TL;DR: The present study associates the early suppression of genes expressed in this host–pathogen interaction with enhancement of R-gene mediated resistance, and identified a chromosome 2H trans-eQTL hotspot that co-segregates with an enhancer of Rpg-TTKSK-mediated, adult plant resistance discovered through the Njoro field trials.
References
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