Journal ArticleDOI
Pb2+ as a Substrate and a Cofactor of a Porphyrin Metalation DNAzyme
TLDR
A DNAzyme named T30695 (sequence: (G3T)4) is reported that can catalyze Zn2+ insertion into three different porphyrins in the presence of Pb2+ as a cofactor, suggesting the importance of P b2+ stabilized G-quadruplex.Abstract:
We herein report a DNAzyme named T30695 (sequence: (G3 T)4 ) that can catalyze Zn2+ insertion into three different porphyrins in the presence of Pb2+ as a cofactor. Meanwhile, T30695 with Pb2+ alone was found to cause a shift in both the fluorescence and UV-vis spectra of protoporphyrin IX (PPIX), thus suggesting that metalation of Pb2+ was also achieved at room temperature. From kinetic measurements, the reaction required two Pb2+ ions; this is consistent with one being a cofactor and the other being a substrate. No previous reports inserted Pb2+ into porphyrins by using DNAzymes or protein-based enzymes. This reaction was most significantly inhibited in the presence of K+ followed by Na+ and Li+ , suggesting the importance of the Pb2+ -stabilized G-quadruplex. When Pb2+ is inserted into PPIX, its emission blue shifts from 635 to 590 nm, thus allowing simple ratiometric fluorescent sensing with a detection limit of 1.2 nM Pb2+ .read more
Citations
More filters
Journal ArticleDOI
G-quadruplex DNA for construction of biosensors
TL;DR: G-quadruplex (G4) DNA has attracted growing interest since it can be used for both target recognition and signal transduction as discussed by the authors, and it has been widely used for its stable chemical properties, cost-effectiveness, and easy modification.
Journal ArticleDOI
Review of recent progress on DNA-based biosensors for Pb2+ detection
TL;DR: A review of Pb2+ recognition mechanisms from fundamental chemistry to the design of fluorescent, colorimetric, and electrochemical biosensors can be found in this article, where various signal amplification mechanisms such as rolling circle amplification, hairpin hybridization chain reaction and nuclease-assisted methods are coupled to these sensing methods to drive up sensitivity.
Journal ArticleDOI
Selection of a metal ligand modified DNAzyme for detecting Ni2.
Wei Ren,Po-Jung Jimmy Huang,Donatien de Rochambeau,Woohyun J. Moon,Jinyi Zhang,Mingsheng Lyu,Shujun Wang,Hanadi F. Sleiman,Juewen Liu +8 more
TL;DR: To increase Ni2+ binding, a glycyl-histidine-functionalized tertiary amine moiety was inserted at the cleavage junction and a catalytic beacon biosensor was designed by labeling a fluorophore and a quencher on the Ni03l DNAzyme.
Journal ArticleDOI
DNAmoreDB, a database of DNAzymes
TL;DR: DNAmoreDB is a comprehensive database resource for DNAzymes that collects and organizes the following types of information: sequences, conditions of the selection procedure, catalyzed reactions, kinetic parameters, substrates, cofactors, structural information whenever available, and literature references.
Journal ArticleDOI
Porphyrin metalation catalyzed by DNAzymes and nanozymes
TL;DR: This review summarizes the progress in DNAzymes and nanozymes due to their excellent stability and low cost and reviews the applications of porphyrin metalation reactions for the detection of various metal ions, improving photocatalytic activity, and removing heavy metal ions in water.
References
More filters
Journal ArticleDOI
Direct evidence for a G-quadruplex in a promoter region and its targeting with a small molecule to repress c-MYC transcription.
TL;DR: The principle that c-MYC transcription can be controlled by ligand-mediated G-quadruplex stabilization is established, establishing the principle that the purine-rich strand of the DNA in this region can form two different intramolecular G- quadruplex structures.
Journal ArticleDOI
Fluorescent and colorimetric sensors for detection of lead, cadmium, and mercury ions
TL;DR: In this critical review, the fluorescent and colorimetric sensors are classified according to their receptors into several categories, including small molecule based sensors, calixarene based Chemosensors, BODIPY based chemosensor, polymer based chemOSensors), DNA functionalized sensing systems, protein based sensing systems and nanoparticle based sensing Systems.
Journal ArticleDOI
Molecular robots guided by prescriptive landscapes
Kyle Lund,Anthony J. Manzo,Dabby Nadine L,Nicole Michelotti,Alexander Johnson-Buck,Jeanette Nangreave,Steven Taylor,Renjun Pei,Milan N. Stojanovic,Nils G. Walter,Erik Winfree,Hao Yan +11 more
TL;DR: It is demonstrated that previously developed random walkers show elementary robotic behaviour when interacting with a precisely defined environment and single-molecule microscopy observations confirm that such walkers achieve directional movement by sensing and modifying tracks of substrate molecules laid out on a two-dimensional DNA origami landscape.
Journal ArticleDOI
DNAzymes for sensing, nanobiotechnology and logic gate applications
TL;DR: The use of catalytic nucleic acids for amplified biosensing was accomplished by designing aptamer-DNAzyme conjugates that combine recognition units and amplifying readout units as in integrated biosensing materials.
Journal ArticleDOI