Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide.
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TLDR
Peroxynitrite anion was a less effective thiol-oxidizing agent than its anion, with oxidation presumably mediated by the decomposition products, hydroxyl radical and nitrogen dioxide.About:
This article is published in Journal of Biological Chemistry.The article was published on 1991-03-05 and is currently open access. It has received 2487 citations till now. The article focuses on the topics: Peroxynitrite & Peroxynitrous acid.read more
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Nitric Oxide and Peroxynitrite in Health and Disease
TL;DR: Current evidence indicates that most of the cytotoxicity attributed to NO is rather due to peroxynitrite, produced from the diffusion-controlled reaction between NO and another free radical, the superoxide anion, which is presented in detail in this review.
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Interferon-γ: an overview of signals, mechanisms and functions
TL;DR: The current understanding of IFN‐γ ligand, receptor, ignal transduction, and cellular effects with a focus on macrophage responses and to a lesser extent, responses from other cell types that influence macrophages function during infection are reviewed.
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Reactive Oxygen Species and the Central Nervous System
TL;DR: The nature of antioxidants is discussed, it being suggested that antioxidant enzymes and chelators of transition metal ions may be more generally useful protective agents than chain‐breaking antioxidants.
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Biochemistry of nitric oxide and its redox-activated forms
TL;DR: The integration of this chemistry with current perspectives of NO biology illuminates many aspects of NO biochemistry, including the enzymatic mechanism of synthesis, the mode of transport and targeting in biological systems, the means by which its toxicity is mitigated, and the function-regulating interaction with target proteins.
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A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds
Stuart A. Lipton,Yun-Beom Choi,Yun-Beom Choi,Zhuo Hua Pan,Zhuo Hua Pan,Sizheng Z. Lei,Sizheng Z. Lei,Huei-Sheng Vincent Chen,Huei-Sheng Vincent Chen,Nikolaus J. Sucher,Nikolaus J. Sucher,Joseph Loscalzo,Joseph Loscalzo,David J. Singel,Jonathan S. Stamler,Jonathan S. Stamler +15 more
TL;DR: It is reported that NO.-mediated neurotoxicity is engendered, at least in part, by reaction with superoxide anion (O.-2), apparently leading to formation of peroxynitrite (ONOO−), and not by NO.
References
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Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
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Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide.
TL;DR: It is proposed that superoxide dismutase may protect vascular tissue stimulated to produce superoxide and NO under pathological conditions by preventing the formation of peroxynitrite.
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Hydroperoxide metabolism in mammalian organs.
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Oxygen-derived free radicals in postischemic tissue injury.
TL;DR: It is now clear that oxygen-derived free radicals play an important part in several models of experimentally induced reperfusion injury, and Dysfunction induced by free radicals may be a major component of ischemic diseases of the heart, bowel, liver, kidney, and brain.