Pinkbar is an epithelial-specific BAR domain protein that generates planar membrane structures
Anette Pykäläinen,Malgorzata Boczkowska,Hongxia Zhao,Juha Saarikangas,Grzegorz Rebowski,Maurice Jansen,Janne Hakanen,Essi V. Koskela,Johan Peränen,Helena Vihinen,Eija Jokitalo,Marjo Salminen,Elina Ikonen,Roberto Dominguez,Pekka Lappalainen +14 more
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TLDR
Structural and mutagenesis analyses reveal that the BAR domain of Pinkbar has a relatively flat lipid-binding interface and that it assembles into sheet-like oligomers in crystals and in solution, which may explain its unique membrane-deforming activity.Abstract:
Bin/amphipysin/Rvs (BAR)-domain proteins sculpt cellular membranes and have key roles in processes such as endocytosis, cell motility and morphogenesis. BAR domains are divided into three subfamilies: BAR- and F-BAR-domain proteins generate positive membrane curvature and stabilize cellular invaginations, whereas I-BAR-domain proteins induce negative curvature and stabilize protrusions. We show that a previously uncharacterized member of the I-BAR subfamily, Pinkbar, is specifically expressed in intestinal epithelial cells, where it localizes to Rab13-positive vesicles and to the plasma membrane at intercellular junctions. Notably, the BAR domain of Pinkbar does not induce membrane tubulation but promotes the formation of planar membrane sheets. Structural and mutagenesis analyses reveal that the BAR domain of Pinkbar has a relatively flat lipid-binding interface and that it assembles into sheet-like oligomers in crystals and in solution, which may explain its unique membrane-deforming activity.read more
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Membrane curvature and its generation by BAR proteins
Carsten Mim,Vinzenz M. Unger +1 more
TL;DR: This work focuses on how BAR proteins interact with the membrane, and how the resulting scaffold structures might aid the recruitment of other proteins to the sites where membranes are bent.
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Artificial photosynthetic cell producing energy for protein synthesis.
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Structural Basis of Membrane Bending by the N-BAR Protein Endophilin
Carsten Mim,Haosheng Cui,Joseph Gawronski-Salerno,Adam Frost,Edward Lyman,Gregory A. Voth,Vinzenz M. Unger +6 more
TL;DR: Coarse-grained molecular dynamics simulations reveal that endophilin lattices are highly dynamic and that the N-terminal helices are required for formation of a stable and regular scaffold, suggesting that the spatial presentation of SH3 domains, rather than affinity, governs the recruitment of downstream interaction partners.
Journal ArticleDOI
Dynamic Shaping of Cellular Membranes by Phospholipids and Membrane-Deforming Proteins
TL;DR: The characteristics of phospholipids, and the mechanisms utilized by phosphoinositides to regulate cellular events are described, which summarize the precise mechanisms underlying the construction of membrane micro-structures and their involvements in physiological and pathological processes.
Journal ArticleDOI
Structures, Functions, and Dynamics of ESCRT-III/Vps4 Membrane Remodeling and Fission Complexes.
TL;DR: Recent advances in the understanding of the structures, activities, and mechanisms of the ESCRT-III and Vps4 machinery are reviewed, including the first high-resolution structures of ESC RT-III filaments, the assembled VPS4 enzyme in complex with an ESCRT/Vps4 substrate, and emerging mechanistic models for ESCRT -mediated membrane fission.
References
More filters
Journal ArticleDOI
Coot: model-building tools for molecular graphics.
Paul Emsley,Kevin Cowtan +1 more
TL;DR: CCP4mg is a project that aims to provide a general-purpose tool for structural biologists, providing tools for X-ray structure solution, structure comparison and analysis, and publication-quality graphics.
Journal ArticleDOI
Computer Visualization of Three-Dimensional Image Data Using IMOD
TL;DR: IMOD is useful for studying and modeling data from tomographic, serial section, and optical section reconstructions and allows image data to be visualized by several different methods.
Journal ArticleDOI
Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability
TL;DR: It is concluded that Caco-2 cells grown on collagen-coated polycarbonate membranes should represent a valuable transport model system for the small intestinal epithelium.
Journal ArticleDOI
Membrane curvature and mechanisms of dynamic cell membrane remodelling
TL;DR: Membrane curvature is no longer seen as a passive consequence of cellular activity but an active means to create membrane domains and to organize centres for membrane trafficking.
Journal ArticleDOI
BAR domains as sensors of membrane curvature: the amphiphysin BAR structure
Brian J. Peter,Helen M. Kent,Ian G. Mills,T. Yvonne Vallis,P. Jonathan G. Butler,Philip R. Evans,Harvey T. McMahon +6 more
TL;DR: The structure of the Drosophila amphiphysin BAR domain is solved and it is predicted that BAR domains are in many protein families, including sorting nexins, centaurins, and oligophrenins.
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