Platelet thrombospondin modulates endothelial cell adhesion, motility, and growth: a potential angiogenesis regulatory factor.
TLDR
Thrombospondin (TSP), an extracellular matrix component, induces adhesion and spreading of murine lung capillary and bovine aortic endothelial cells and has the potential to modulate the angiogenic process.Abstract:
Components of the extracellular matrix have been shown to modulate the interaction of endothelial cells with their microenvironment. Here we report that thrombospondin (TSP), an extracellular matrix component, induces adhesion and spreading of murine lung capillary (LE-II) and bovine aortic (BAEC) endothelial cells. This TSP-induced spreading was inhibited by heparin and fucoidan, known to bind the amino-terminal globular domain of the molecule. In addition, endothelial cells were induced to migrate by a gradient of soluble TSP (chemotaxis). The chemotactic response was inhibited by heparin and fucoidan, as well as by the mAb A2.5, which also binds to the amino-terminal domain. These data are in agreement with our previous observation that the TSP aminoterminal heparin binding region is responsible for the induction of tumor cell spreading and chemotactic motility. The inhibition of chemotaxis and spreading by antibodies against the beta 3 but not the beta 1 chain of the integrin receptor points to a role for the integrins in the interaction of endothelial cells with TSP. We also found that TSP modulates endothelial cell growth. When added to quiescent LE-II cells, it inhibited the mitogenic effects of serum and the angiogenic factor bFGF, in a dose-dependent manner. The inhibition of DNA synthesis detected in the mitogenic assay resulted in a true inhibition of BAEC and LE-II cell growth, as assessed by proliferation assay. This work indicates that TSP affects endothelial cell adhesion, spreading, motility and growth. TSP, therefore, has the potential to modulate the angiogenic process.read more
Citations
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Nitric oxide synthase lies downstream from vascular endothelial growth factor-induced but not basic fibroblast growth factor-induced angiogenesis.
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CD36 Mediates the In Vitro Inhibitory Effects of Thrombospondin-1 on Endothelial Cells
David W. Dawson,S. Frieda A. Pearce,Ruiqin Zhong,Roy L. Silverstein,William A. Frazier,Noel P. Bouck +5 more
TL;DR: This work demonstrates that endothelial CD36, previously thought to be involved only in adhesion and scavenging activities, may be essential for the inhibition of angiogenesis by thrombospondin-1.
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Peptides derived from two separate domains of the matrix protein thrombospondin-1 have anti-angiogenic activity
Sara S. Tolsma,Olga V. Volpert,Deborah J. Good,William A. Frazier,Peter J. Polverini,Noel P. Bouck +5 more
TL;DR: The results suggest that the large TSP1 molecule employs at least two different structural domains and perhaps two different mechanisms to accomplish a single physiological function, the inhibition of neovascularization.
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Extracellular proteins that modulate cell-matrix interactions. SPARC, tenascin, and thrombospondin.
E H Sage,Paul Bornstein +1 more
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TL;DR: An 88-kD membrane glycoprotein present in platelets, endothelial cells, monocytes, and a variety of human tumor cell lines that is the membrane binding site for TSP is identified and isolated and may function as the cellular TSP receptor.