Isolation of the thrombospondin membrane receptor.
TLDR
An 88-kD membrane glycoprotein present in platelets, endothelial cells, monocytes, and a variety of human tumor cell lines that is the membrane binding site for TSP is identified and isolated and may function as the cellular TSP receptor.Abstract:
Thrombospondin (TSP), a 450-kD multifunctional glycoprotein with a broad tissue distribution, is secreted upon platelet stimulation, binds to the activated platelet surface, and supports platelet aggregation. We have identified and isolated an 88-kd membrane glycoprotein present in platelets, endothelial cells, monocytes, and a variety of human tumor cell lines that is the membrane binding site for TSP. Endogenous platelet TSP binding to thrombin- and ionophore-stimulated human platelets was inhibited in the presence of the monoclonal antibody OKM5. TSP binding to C32 melanoma cells and HT1080 fibrosarcoma cells was specific and also inhibitable with OKM5 Mab. Cell labeling followed by specific immunoprecipitation demonstrated biosynthesis of a single 88-kD glycoprotein. Binding of TSP to the isolated membrane protein was specific and saturable. These studies identify an 88-kD membrane glycoprotein that reacts with the monoclonal antibody, OKM5, and may function as the cellular TSP receptor.read more
Citations
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PPARγ Promotes Monocyte/Macrophage Differentiation and Uptake of Oxidized LDL
Peter Tontonoz,Laszlo Nagy,Laszlo Nagy,Jacqueline G.A Alvarez,Vilmos A. Thomazy,Ronald M. Evans,Ronald M. Evans +6 more
TL;DR: It is demonstrated that the nuclear receptor PPARγ is induced in human monocytes following exposure to oxLDL and is expressed at high levels in the foam cells of atherosclerotic lesions, and it is suggested that endogenous PParγ ligands may be important regulators of gene expression during atherogenesis.
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Inferences, questions and possibilities in Toll-like receptor signalling
TL;DR: The Toll-like receptors are the key proteins that allow mammals — whether immunologically naive or experienced — to detect microbes and many inflammatory processes, both sterile and infectious, may depend on TLR signalling.
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CD36, a Scavenger Receptor Involved in Immunity, Metabolism, Angiogenesis, and Behavior
TL;DR: CD36 binds to several major classes of ligands, including the matrix protein thrombospondin, long-chain fatty acids, and oxidized phospholipids and lipoproteins; in different contexts, it serves to regulate angiogenesis, innate immune responses, fatty acid metabolism, and sensory responses to fatty acids.
Journal ArticleDOI
Expression cloning of SR-BI, a CD36-related class B scavenger receptor.
TL;DR: SR-BI and CD36 define a second class of scavenger receptors, designated class B, whose predicted protein sequence of 509 amino acids is approximately 30% identical to those of the four previously identified family members.
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Thrombospondin cooperates with CD36 and the vitronectin receptor in macrophage recognition of neutrophils undergoing apoptosis.
TL;DR: Newly defined roles for TSP and CD36 in phagocytic clearance of senescent neutrophils, which may limit inflammatory tissue injury and promote resolution are indicated.
References
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