Book ChapterDOI
Preparation of Impermeable Inside-Out and Right-Side-Out Vesicles from Erythrocyte Membranes
Theodore L. Steck
- pp 245-281
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TLDR
In this paper, it is shown that each biological membrane operates differentially on the two compartments it separates and is thus anisotropic in its function, and that the molecular constituents of the two surfaces differ and that this asymmetry constitutes a principal feature of membrane organization.Abstract:
Each biological membrane operates differentially on the two compartments it separates and is thus anisotropic in its function. It is reasonable to suppose that the molecular constituents of the two surfaces differ and that this asymmetry constitutes a principal feature of membrane organization. Since membranes are only a few macromolecules thick, it is clear that enumeration of the components at each surface would do much to define the structure as a whole.read more
Citations
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Journal ArticleDOI
THE ORGANIZATION OF PROTEINS IN THE HUMAN RED BLOOD CELL MEMBRANE A Review
TL;DR: This article will focus on the localization and modes of association of individual major polypeptides within the human red cell membrane.
Journal ArticleDOI
Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane
TL;DR: Band 3 is the major, membrane-spanning, approximately90 000 dalton polypeptide of the human erythrocyte membrane and an interchain disulfide bond, which can be induced only at the cytoplasmic surface, cross-linked intact band 3, and certain of its water-soluble fragments.
Journal ArticleDOI
Membrane asymmetry and blood coagulation
TL;DR: It is described here experiments devised to show that the asymmetric distribution of membrane phospholipids in blood cells may serve a biological purpose, by contributing to maintain the delicate balance between regulating haemostasis and avoiding thrombosis.
Journal ArticleDOI
Rat liver proteins capable of transferring phosphatidylethanolamine. Purification and transfer activity for other phospholipids and cholesterol.
B Bloj,D B Zilversmit +1 more
TL;DR: The fraction obtained after ammonium sulfate precipitation, gel filtration on Sephadex G-75, ion-exchange chromatography on CM-cellulose, ampholyte displacement chromatography, and heat treatment exhibited an 876-fold increase in its phosphatidylethanolamine transfer activity as compared with the postmitochondrial supernatant adjusted to pH 5.1.
Journal ArticleDOI
The Molecular Basis of Erythrocyte Shape
TL;DR: The erythrocyte is the first nonmuscle cell for which it will be possible to extend the knowledge of molecular interactions to the next hierarchical level of organization that deals with shape and shape transformations.
References
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Journal ArticleDOI
A new and rapid colorimetric determination of acetylcholinesterase activity.
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.
Journal ArticleDOI
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes
TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.