Production of Biodiesel from Acid Oil via a Two-Step Enzymatic Transesterification.
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TLDR
A two-step enzymatic transesterification process in a solvent-free system has been developed as a novel approach to the production of biodiesel using acid oil from rice bran oil soapstock with maximum yields obtained in both stepwise uses.Abstract:
A two-step enzymatic transesterification process in a solvent-free system has been developed as a novel approach to the production of biodiesel using acid oil from rice bran oil soapstock. The acid oil consisted of 53.7 wt% fatty acids, 2.4 wt% monoacylglycerols, 9.1 wt% diacylglycerols, 28.8 wt% triacylglycerols, and 6.0 wt% others. Three immobilized lipases were evaluated as potential biocatalysts, including Novozym 435 from Candida antarctica, Lipozyme RM IM from Rhizomucor miehei, and Lipozyme TL IM from Thermomyces lanuginosus. The effects of molar ratio of acid oil to ethanol, temperature, and enzyme loading were investigated to determine the optimum conditions for the transesterification with the three immobilized lipases. The optimum conditions of the three immobilized lipases were a molar ratio of 1:5 (acid oil to ethanol), the temperature range of 30-40°C, and the enzyme loading range of 5-10%. The two-step transesterification was then conducted under the optimum conditions of each lipase. The stepwise use of Novozym 435 and Lipozyme TL IM or Lipozyme RM IM and Lipozyme TL IM resulted in similar or higher levels of yield to the individual lipases. The maximum yields obtained in both stepwise uses were ca. 92%.read more
Citations
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Journal ArticleDOI
Novozym 435: the “perfect” lipase immobilized biocatalyst?
Claudia Ortiz,María Luján Ferreira,Oveimar Barbosa,José C. S. dos Santos,Rafael C. Rodrigues,Ángel Berenguer-Murcia,Laura E. Briand,Roberto Fernandez-Lafuente +7 more
TL;DR: Novozym 435 (N435) is a commercially available immobilized lipase produced by Novozymes with its advantages and drawbacks.
Journal ArticleDOI
Scale-up of biodiesel synthesis in a closed-loop packed-bed bioreactor system using the fermented solid produced by Burkholderia lata LTEB11
TL;DR: In this paper, the authors used bi-distilled olein as the source of fatty acids and achieved a molar ratio of ethanol to fatty acids of 1.5:1, in a process in which 245 g of ethanol and 1000 g of olein were circulated in a closed-loop system through a column containing 120 g of dry fermented solids.
Book ChapterDOI
Production of biodiesel from rice bran oil
Dayang Norulfairuz Abang Zaidel,Ida Idayu Muhamad,Nurul Shafinas Mohd Daud,Nor Azyati Abdul Muttalib,Nozieana Khairuddin,Nurul Asmak Md Lazim +5 more
TL;DR: Rice bran is a byproduct of the milling process of paddy rice to produce refined rice and is a valuable domestic oil resource in many countries, but its acceptability as an edible oil has been hampered because of its commonly high free fatty acid (FFA) content resulting from the high lipase activity of the bran as mentioned in this paper.
Journal ArticleDOI
Recent Advances in Feedstock and Lipase Research and Development towards Commercialization of Enzymatic Biodiesel
TL;DR: In this paper, the authors reviewed the recent progress in feedstock development, including first, second, third, and fourth generation feedstocks for biodiesel production, and discussed recent progress of lipase research and development as one of the key factors for establishing a cost-competitive biodiesel process in terms of enzyme sources, properties, immobilization, and transesterification efficiency.
Journal ArticleDOI
Two novel lipases purified from rice bran displaying lipolytic and esterification activities.
TL;DR: Two novel lipases, RBL1 and RBL2, are different from published native lipases in rice bran and may be alternative potential candidates of biocatalysts contributing to the development of diverse industrial application fields.
References
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Biodiesel production : a review
Fangrui Ma,Milford A. Hanna +1 more
TL;DR: In this article, the transesterification reaction is aected by molar ratio of glycerides to alcohol, catalysts, reaction temperature, reaction time and free fatty acids and water content of oils or fats.
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TL;DR: The current status of biodiesel production with immobilized lipase is reviewed, including various lipases, immobilization methods, various feedstocks, lipase inactivation caused by short chain alcohols and large scale industrialization.
Journal ArticleDOI
Comparative study on lipase-catalyzed transformation of soybean oil for biodiesel production with different acyl acceptors
TL;DR: Methyl acetate, a novel acyl acceptor for biodiesel production, seems very promising for lipase-catalyzed large-scale production of biodiesel, and the by-product triacetylglycerol is an important chemical with a higher value than glycerol.
Journal ArticleDOI
Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent
TL;DR: It was found, using a batch system, that enzyme inhibition caused by undissolved methanol was eliminated by adding t-butanol to the reaction medium, which also gave a noticeable increase of reaction rate and ester yield.