Journal ArticleDOI
Production of nephrotic syndrome in rats by Freund's adjuvants and rat kidney suspensions.
TLDR
The small amounts of rat kidney proteins used, support the interpretation that an isoand autosensitization mechanism is at play, and help clarify the mechanism behind the severe nephrotic syndrome noted in 20 rats given Freund's adjuvants and a supernate of rat kidneys suspension by intraperitoneal injection.Abstract:
Summary1) A severe nephrotic syndrome was noted in 20 rats given Freund's adjuvants and a supernate of rat kidney suspension by intraperitoneal injection. When kidney tissue was replaced by liver, only 3 of 21 animals developed a much milder renal disease. Lung or muscle suspensions failed to induce proteinuria in 10 rats. 2) In large doses (0.5 ml) Freund's adjuvants alone produced a mild nephrotic disease in 3 of 10 rats. Even though the smaller amounts of the adjuvants usually used (0.25 ml) never produced proteinuria, slight histological alterations of the glomerular structure were frequently noted. 3) In contradistinction to other tissue suspensions addition of rat kidney supernate enhanced the effect of adjuvants markedly and regularly. 4) The small amounts of rat kidney proteins used, support the interpretation that an isoand autosensitization mechanism is at play.read more
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Journal ArticleDOI
Target antigens and nephritogenic antibodies in membranous nephropathy: of rats and men.
Pierre Ronco,H Debiec +1 more
TL;DR: Substantial progresses have been made in understanding molecular mechanisms of membranous nephropathy, which should lead to novel therapeutic approaches, and the role of alloimmunization as a new mechanism of renal disease is discussed.
Journal ArticleDOI
Immunological consequences of using three different clinical/laboratory techniques of emulsifying peptide-based vaccines in incomplete Freund's adjuvant
TL;DR: All three techniques can be used to prepare peptide-based vaccine emulsions under optimal dose conditions and investigators are urged to take special care in their choice of mixing vessels for the vortex technique as that can influence the stability of the emulsion.
Journal ArticleDOI
Possible involvement of terminal complement complex in active Heymann nephritis
TL;DR: The detection of the C5b-9 complex of complement in glomerular deposits coincided with the development of abnormal proteinuria indicating that the glomersular damage in this autoimmune disease may be caused by complement-mediated lesions in the glomerul capillary walls.