Journal ArticleDOI
Protein disulfide engineering.
TLDR
Progress in disulfide engineering is reviewed, with an emphasis on the issue of stability and computational methods that facilitate engineering efforts.About:
This article is published in FEBS Letters.The article was published on 2014-01-21. It has received 205 citations till now.read more
Citations
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Journal ArticleDOI
Functional and Molecular Characterization of the Halomicrobium sp. IBSBa Inulosucrase.
Gülbahar Abaramak,Jaime Ricardo Porras-Domínguez,Henry Christopher Janse van Rensburg,Eveline Lescrinier,Ebru Toksoy Oner,Onur Kırtel,Wim Van den Ende +6 more
TL;DR: In this paper, the first in-depth functional and molecular characterization of an Archaeal inulosucrase from Halomicrobium sp. IBSBa (HmcIsc) was described.
Book ChapterDOI
Thermophiles and thermophilic hydrolases
TL;DR: Thermophilic enzymes, the thermophilic hydrolases involved in depolymerization of biopolymers, such as xylanases, proteases, cellulases, amylases, and lipases, are of special interest due to their applications in food, pharmaceutical, pulp and paper industries and environmental biotechnology.
Journal ArticleDOI
Computational Modeling of the Disulfide Cross-Linking Reaction.
TL;DR: Computer simulation of the disulfide cross-linking reaction with hydrogen peroxide was performed at the integrated quantum mechanical/molecular mechanical (QM/MM) level of theory in a water box under periodic boundary conditions, and the solvent-assisted proton-exchange/proton-transfer effects were examined on the energetic barriers for the different transition states.
Book ChapterDOI
Bioremediation of Polluted Soil by Using Plant Growth–Promoting Rhizobacteria
TL;DR: In this paper, many rhizosphere microorganisms including Azotobacter spp., Pseudomonas aeruginosa, Glomus spp. have been found as plant growth-promoting rhizobacteria.
Journal ArticleDOI
Tuning Enzyme Thermostability via Computationally Guided Covalent Stapling and Structural Basis of Enhanced Stabilization
Jacob A Iannuzzelli,John-Paul Bacik,Eric Moore,Zhuofan Shen,Ellen M Irving,David A. Vargas,Sagar D. Khare,Nozomi Ando,Rudi Fasan +8 more
TL;DR: This work demonstrates the functionality of an expanded set of electrophilic amino acids featuring chloroacetamido, acrylamido, and vinylsulfonamido side-chain groups for protein stapling using a myoglobin-based cyclopropanase as a model enzyme and expands the toolbox of proteinStapling strategies available for protein stabilization.
References
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Book
Proteins: Structures and Molecular Properties
TL;DR: This paper discusses the physical properties of polypeptides, the structure of which has been determined Crystallographically to High Resolution and its role in the biosynthesis of Proteins.
Journal ArticleDOI
Atomic-Level Characterization of the Structural Dynamics of Proteins
David E. Shaw,David E. Shaw,Paul Maragakis,Kresten Lindorff-Larsen,Stefano Piana,Ron O. Dror,Michael P. Eastwood,Joseph A. Bank,John M. Jumper,John K. Salmon,Yibing Shan,Willy Wriggers +11 more
TL;DR: Simulation of the folding of a WW domain showed a well-defined folding pathway and simulation of the dynamics of bovine pancreatic trypsin inhibitor showed interconversion between distinct conformational states.
Journal ArticleDOI
Molecular dynamics: survey of methods for simulating the activity of proteins.
TL;DR: This review offers an outline of the origin of molecular dynamics simulation for protein systems and how it has developed into a robust and trusted tool, and covers more recent advances in theory and an illustrative selection of practical studies in which it played a central role.
Journal ArticleDOI
Molecular dynamics simulations in biology.
Martin Karplus,Gregory A. Petsko +1 more
TL;DR: Molecular dynamics is also being used to determine protein structures from NMR, to refine protein X-ray crystal structures faster from poorer starting models, and to calculate the free energy changes resulting from mutations in proteins.
Journal ArticleDOI
Disulphide bridges in globular proteins
TL;DR: There is a strong preference for shorter connections, with half-cystines separated by less than 24 residues in 49% of all disulphides.