Journal ArticleDOI
Protein disulfide engineering.
TLDR
Progress in disulfide engineering is reviewed, with an emphasis on the issue of stability and computational methods that facilitate engineering efforts.About:
This article is published in FEBS Letters.The article was published on 2014-01-21. It has received 205 citations till now.read more
Citations
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Journal ArticleDOI
Forces stabilizing proteins
TL;DR: What has been learned about the major forces stabilizing proteins since the late 1980s when site‐directed mutagenesis became possible is summarized.
Journal ArticleDOI
Review: Engineering of thermostable enzymes for industrial applications
TL;DR: The most relevant enzymes that are used for industrial applications are provided and the strategies that are adopted to enhance enzyme stability and/or activity are discussed, along with some of the most relevant achievements.
Journal ArticleDOI
Protein stability: a crystallographer's perspective.
TL;DR: This review will focus on factors affecting protein stability on a somewhat practical level, particularly from the view of a protein crystallographer.
Journal ArticleDOI
Principles of Protein Stability and Their Application in Computational Design
TL;DR: The computational stability design methods have advanced over the past two decades starting from methods that selectively addressed only some aspects of marginal stability, such as thermodynamic, cellular, and evolutionary principles and mechanisms that underlie marginal stability as mentioned in this paper.
Journal ArticleDOI
FoldX as Protein Engineering Tool: Better Than Random Based Approaches?
TL;DR: In this review different algorithms for the prediction of beneficial mutation sites to enhance protein stability are summarized and the advantages and disadvantages of FoldX are highlighted.
References
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Journal ArticleDOI
Lessons from the lysozyme of phage T4
TL;DR: An overview is presented of some of the major insights that have come from studies of the structure, stability, and folding of T4 phage lysozyme, including melting temperatures, crystallographic data, Protein Data Bank access codes, and references to the original literature.
Journal ArticleDOI
Stereochemical modeling of disulfide bridges. Criteria for introduction into proteins by site-directed mutagenesis
Ramanathan Sowdhamini,Narayanaswamy Srinivasan,Brian K. Shoichet,Daniel V. Santi,C. Ramakrishnan,Padmanabhan Balaram +5 more
TL;DR: A computer modeling procedure for assessing the stereochemical suitability of pairs of residues in proteins as potential sites for introduction of cystine disulfide crosslinks has been developed and two positions for the introduction of 'stereochemically optimal' disulfides are identified in subtilisin.
Journal ArticleDOI
Engineering proteins for thermostability through rigidifying flexible sites.
Haoran Yu,He Huang +1 more
TL;DR: An overview of a strategy proven effective in enhancing proteins' thermostability with two steps: predict flexible sites of proteins firstly and then rigidify these sites (RFS) is given.
Journal ArticleDOI
Extreme Stabilization of a Thermolysin-like Protease by an Engineered Disulfide Bond
Johanna Mansfeld,Gert Vriend,Bauke W. Dijkstra,O. Rob Veltman,Bertus van den Burg,Gerard Venema,Renate Ulbrich-Hofmann,Vincent G. H. Eijsink +7 more
TL;DR: It is proposed that the success of the present stabilization strategy is the result of the localization and mutation of an area of the molecule involved in the partial unfolding processes that determine thermal stability.
Journal ArticleDOI
Improved amino acid flexibility parameters
TL;DR: It was found that the distribution of normalized B‐factors followed a Gumbel, or extreme value distribution, and the location parameter, or mode, of this distribution was used as an estimator of flexibility for the amino acid.