Journal ArticleDOI
Protein disulfide engineering.
TLDR
Progress in disulfide engineering is reviewed, with an emphasis on the issue of stability and computational methods that facilitate engineering efforts.About:
This article is published in FEBS Letters.The article was published on 2014-01-21. It has received 205 citations till now.read more
Citations
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Journal ArticleDOI
Forces stabilizing proteins
TL;DR: What has been learned about the major forces stabilizing proteins since the late 1980s when site‐directed mutagenesis became possible is summarized.
Journal ArticleDOI
Review: Engineering of thermostable enzymes for industrial applications
TL;DR: The most relevant enzymes that are used for industrial applications are provided and the strategies that are adopted to enhance enzyme stability and/or activity are discussed, along with some of the most relevant achievements.
Journal ArticleDOI
Protein stability: a crystallographer's perspective.
TL;DR: This review will focus on factors affecting protein stability on a somewhat practical level, particularly from the view of a protein crystallographer.
Journal ArticleDOI
Principles of Protein Stability and Their Application in Computational Design
TL;DR: The computational stability design methods have advanced over the past two decades starting from methods that selectively addressed only some aspects of marginal stability, such as thermodynamic, cellular, and evolutionary principles and mechanisms that underlie marginal stability as mentioned in this paper.
Journal ArticleDOI
FoldX as Protein Engineering Tool: Better Than Random Based Approaches?
TL;DR: In this review different algorithms for the prediction of beneficial mutation sites to enhance protein stability are summarized and the advantages and disadvantages of FoldX are highlighted.
References
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Journal ArticleDOI
Folding the proteome.
TL;DR: New methods that can quantify the folding behavior of a truly broad set of proteins under in vivo conditions will provide a more comprehensive framework from which to understand the connections between protein folding, the molecular basis of disease, and cell function and evolution.
Journal ArticleDOI
Analysis of temperature factor distribution in high‐resolution protein structures
TL;DR: The temperature factors obtained from X‐ray refinement of proteins at high resolution show large variations from one structure to another, but the B‐values expressed in units of standard deviation about their mean value (B'‐factor) at the Cα atoms show remarkably characteristic frequency distribution.
Journal ArticleDOI
Concerted Action of the Ribosome and the Associated Chaperone Trigger Factor Confines Nascent Polypeptide Folding
Anja Hoffmann,Annemarie H. Becker,Beate Zachmann-Brand,Elke Deuerling,Bernd Bukau,Günter Kramer +5 more
TL;DR: These ribosome- and Trigger Factor-specific activities together constitute an efficient mechanism to prevent or even revert premature folding, effectively limiting misfolded intermediates during protein synthesis.
Journal ArticleDOI
MODIP revisited: re-evaluation and refinement of an automated procedure for modeling of disulfide bonds in proteins
TL;DR: The stereochemical criteria used by MODIP for the selection and gradation of sites for modeling disulfides were re-evaluated and steric criteria were introduced to check for energetically unfavorable non-bonded contacts with the modeled disulfide.
Journal ArticleDOI
Study and design of stability in GH5 cellulases.
TL;DR: The results indicated that the protein flexibility and optimum activity temperatures are appreciably correlated and factors that are possibly involved in higher rigidity of thermostable cellulases were investigated.