Journal ArticleDOI
Protein disulfide engineering.
TLDR
Progress in disulfide engineering is reviewed, with an emphasis on the issue of stability and computational methods that facilitate engineering efforts.About:
This article is published in FEBS Letters.The article was published on 2014-01-21. It has received 205 citations till now.read more
Citations
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Journal ArticleDOI
Forces stabilizing proteins
TL;DR: What has been learned about the major forces stabilizing proteins since the late 1980s when site‐directed mutagenesis became possible is summarized.
Journal ArticleDOI
Review: Engineering of thermostable enzymes for industrial applications
TL;DR: The most relevant enzymes that are used for industrial applications are provided and the strategies that are adopted to enhance enzyme stability and/or activity are discussed, along with some of the most relevant achievements.
Journal ArticleDOI
Protein stability: a crystallographer's perspective.
TL;DR: This review will focus on factors affecting protein stability on a somewhat practical level, particularly from the view of a protein crystallographer.
Journal ArticleDOI
Principles of Protein Stability and Their Application in Computational Design
TL;DR: The computational stability design methods have advanced over the past two decades starting from methods that selectively addressed only some aspects of marginal stability, such as thermodynamic, cellular, and evolutionary principles and mechanisms that underlie marginal stability as mentioned in this paper.
Journal ArticleDOI
FoldX as Protein Engineering Tool: Better Than Random Based Approaches?
TL;DR: In this review different algorithms for the prediction of beneficial mutation sites to enhance protein stability are summarized and the advantages and disadvantages of FoldX are highlighted.
References
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Journal ArticleDOI
The location of an engineered inter-subunit disulfide bond in factor for inversion stimulation (FIS) affects the denaturation pathway and cooperativity.
TL;DR: Results show that, unlike the C30-C30 intermolecular disulfide bond, the C58-C58 disulfIDE bond did not evenly stabilize the FIS structure, thereby highlighting the importance of the location of an engineered disulfides bond on the propagation of stability and the denaturation cooperativity of a protein.
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GeoFold: Topology-based protein unfolding pathways capture the effects of engineered disulfides on kinetic stability
Vibin Ramakrishnan,Saipraveen Srinivasan,Saeed Salem,Suzanne J. Matthews,Wilfredo Colón,Mohammed J. Zaki,Christopher Bystroff +6 more
TL;DR: GeoFold is a useful tool for the inference of the effects of disulfide engineering on the energy landscape of protein unfolding, and its detailed unfolding pathways for each case explain the results of changes in the chain topology on the folding energy landscape.
Journal ArticleDOI
Engineering monomeric streptavidin and its ligands with infinite affinity in binding but reversibility in interaction.
TL;DR: A pair of engineered biomaterials with the potential to extend the biotin‐streptavidin technology to develop reusable biosensor/protein chips and bioreactors are designed through molecular modeling.
Journal ArticleDOI
Step-wise addition of disulfide bridge in firefly luciferase controls color shift through a flexible loop: a thermodynamic perspective
TL;DR: Thermodynamic analysis revealed that among mutants, L(306)C-L(309)C shows a remarkable stability against urea denaturation and also a considerable increase in kinetic stability and a clear shift in bioluminescence spectra towards red.
Journal ArticleDOI
Energetics of the disulfide bridge: An ab initio study
Weili Qian,Samuel Krimm +1 more
TL;DR: In this article, the energy properties of disulfide bridges have been investigated at the 3-21G* level and at every - 30" in X: and X;, and the additional energies for small AX3 were obtained.