Open AccessJournal Article
Quantitative molecular analysis in mantle cell lymphoma.
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It is demonstrated that detection of the cyclin D1 mRNA level is a molecular marker in 98% of patients with MCL and the quantitative PCR-based analysis is a useful, reliable, rapid, reproducible, sensitive and specific method broadening the authors' diagnostic tools in hematopathology.Abstract:
A molecular analysis has three major roles in modern oncopathology--as an aid in the differential diagnosis, in molecular monitoring of diseases, and in estimation of the potential prognosis. In this report we review the application of the molecular analysis in a group of patients with mantle cell lymphoma (MCL). We demonstrate that detection of the cyclin D1 mRNA level is a molecular marker in 98% of patients with MCL. Cyclin D1 quantitative monitoring is specific and sensitive for the differential diagnosis and for the molecular monitoring of the disease in the bone marrow. Moreover, the dynamics of cyclin D1 in bone marrow reflects the disease development and it predicts the clinical course. We employed the molecular analysis for a precise quantitative detection of proliferation markers, Ki-67, topoisomerase IIalpha, and TPX2, that are described as effective prognostic factors. Using the molecular approach it is possible to measure the proliferation rate in a reproducible, standard way which is an essential prerequisite for using the proliferation activity as a routine clinical tool. Comparing with immunophenotyping we may conclude that the quantitative PCR-based analysis is a useful, reliable, rapid, reproducible, sensitive and specific method broadening our diagnostic tools in hematopathology. In comparison to interphase FISH in paraffin sections quantitative PCR is less technically demanding and less time-consuming and furthermore it is more sensitive in detecting small changes in the mRNA level. Moreover, quantitative PCR is the only technology which provides precise and reproducible quantitative information about the expression level. Therefore it may be used to demonstrate the decrease or increase of a tumor-specific marker in bone marrow in comparison with a previously aspirated specimen. Thus, it has a powerful potential to monitor the course of the disease in correlation with clinical data.read more
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Dissertation
Diversity and function of microbial communities in the Arctic Ocean
TL;DR: In this paper, the diversity and function of bacterial communities in the Arctic Ocean has been explored, despite their often important role in biogeochemical cycling, and the underlying genomic features that deep-sea surface sediment bacteria use for carbon turnover were analyzed, as the degradation of organic matter by heterotrophic bacteria in deep sea sediments regulates the efficiency of CO2 removal from the atmosphere over geological time scales.
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Journal ArticleDOI
A novel cyclin encoded by a bcl1 -linked candidate oncogene
Toru Motokura,Theodora Bloom,Hyung Goo Kim,Harald Jüppner,Joan V. Ruderman,Henry M. Kronenberg,Andrew Arnold +6 more
TL;DR: The data suggest that PRAD1 encodes a novel cyclin whose overexpression may play an important part in the development of various tumours with abnormalities in 11q13.
Journal ArticleDOI
The proliferation gene expression signature is a quantitative integrator of oncogenic events that predicts survival in mantle cell lymphoma
Andreas Rosenwald,George E. Wright,Adrian Wiestner,Wing C. Chan,Wing C. Chan,Joseph M. Connors,Joseph M. Connors,Elias Campo,Elias Campo,Randy D. Gascoyne,Randy D. Gascoyne,Thomas M. Grogan,Thomas M. Grogan,H. Konrad Muller-Hermelink,H. Konrad Muller-Hermelink,Erlend B. Smeland,Erlend B. Smeland,Michael Chiorazzi,Jena M. Giltnane,Elaine M. Hurt,Hong Zhao,Lauren Averett,Sarah E. Henrickson,Liming Yang,John Powell,Wyndham H. Wilson,Elaine S. Jaffe,Richard M. Simon,Richard D. Klausner,Emilio Montserrat,Emilio Montserrat,Francesc Bosch,Francesc Bosch,Timothy C. Greiner,Dennis D. Weisenburger,Dennis D. Weisenburger,Warren G. Sanger,Bhavana J. Dave,James C. Lynch,James C. Lynch,Julie M. Vose,James O. Armitage,Richard I. Fisher,Richard I. Fisher,Thomas P. Miller,Thomas P. Miller,Michael LeBlanc,Michael LeBlanc,German Ott,German Ott,Stein Kvaløy,Stein Kvaløy,Harald Holte,Harald Holte,Jan Delabie,Jan Delabie,Louis M. Staudt +56 more
TL;DR: A quantitative model of the aberrant cell cycle regulation in MCL is proposed that provides a rationale for the design of cell cycle inhibitor therapy in this malignancy.
Journal ArticleDOI
A new prognostic index (MIPI) for patients with advanced-stage mantle cell lymphoma
Eva Hoster,Martin Dreyling,Wolfram Klapper,Christian Gisselbrecht,Achiel Van Hoof,Hanneke C. Kluin-Nelemans,Michael Pfreundschuh,Marcel Reiser,Bernd Metzner,H. Einsele,Norma Peter,Wolfram Jung,Bernhard Wörmann,Wolf-Dieter Ludwig,Ulrich Dührsen,Hartmut Eimermacher,Hannes Wandt,Joerg Hasford,Wolfgang Hiddemann,Michael Unterhalt +19 more
TL;DR: The MIPI as discussed by the authors is the first prognostic index particularly suited for mantle cell lymphoma patients and may serve as an important tool to facilitate risk-adapted treatment decisions in patients with advanced stage MCL.
Journal ArticleDOI
Detection of minimal residual disease in hematologic malignancies by real-time quantitative PCR: principles, approaches, and laboratory aspects.
V H J van der Velden,Andreas Hochhaus,Giovanni Cazzaniga,Tomasz Szczepański,Jean Gabert,J. J. M. Van Dongen +5 more
TL;DR: The interpretation of RQ-PCR MRD data needs standardized criteria and reporting of MRDData needs international uniformity, and several European networks have now been established and common guidelines for data analysis and for reporting ofMRD data are being developed.
Book ChapterDOI
Mantle-cell lymphoma.
TL;DR: The goal of this study was to analyze the presenting features, natural history, and prognostic factors in 59 patients with well characterized mantle cell lymphoma (MCL).