Reaction of the adenine nucleotide analogue 5'-p-fluorosulfonylbenzoyl adenosine at distinct tyrosine and cysteine residues of rabbit muscle pyruvate kinase.
A E Annamalai,R F Colman +1 more
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This article is published in Journal of Biological Chemistry.The article was published on 1981-10-25 and is currently open access. It has received 56 citations till now. The article focuses on the topics: Pyruvate dehydrogenase kinase & Adenine nucleotide.read more
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Sulfonyl fluorides as privileged warheads in chemical biology
Arjun Narayanan,Lyn H. Jones +1 more
TL;DR: The use of sulfonyl fluoride probes in chemical biology is reviewed and three new probes are introduced that provide new insights into the mechanism behind the formation of fluoride in seawater.
Journal ArticleDOI
The BCR gene encodes a novel serine/threonine kinase activity within a single exon
Yoshiro Maru,Owen N. Witte +1 more
TL;DR: The recent demonstration of a GTPase-activating function within the C-terminal portion of BCR suggests that the protein kinase and SH2-binding domains may work in concert with other regions of the molecule in intracellular signalling processes.
Journal ArticleDOI
A study of the reactivity of S(VI)-F containing warheads with nucleophilic amino-acid side chains under physiological conditions.
Herschel Mukherjee,J. Debreczeni,J. Breed,Sharon Tentarelli,Brian Aquila,James E. Dowling,Adrian Whitty,Neil P. Grimster +7 more
TL;DR: The results demonstrate that the reactivity of SFs complements that of more commonly studied acrylamides, and it is hoped that this work spurs the rational design of novel SF-containing covalent probe compounds and inhibitors, particularly in cases where a suitably positioned cysteine residue is not present.
Journal ArticleDOI
Functional identity of a primer recognition protein as phosphoglycerate kinase.
TL;DR: Upon tryptic digestion, amino acid sequencing of tryptic peptides, and homology search against a protein sequence data base, PRP 2 is identified to be the glycolytic enzyme, phosphoglycerate kinase (PGK), and the activities of PRP and PGK increase coordinately in the PRP purification procedure.
Journal ArticleDOI
Inhibition of Bcr serine kinase by tyrosine phosphorylation.
Jiaxin Liu,Yun Wu,Guo Zhen Ma,Dai Lu,Leena Haataja,Nora Heisterkamp,John Groffen,Ralph B. Arlinghaus +7 more
TL;DR: It is reported that in addition to tyrose 177 (Y-177), Y-360 and Y283 are phosphorylated in Bcr-Abl proteins in vitro, and that in Ph-positive leukemia, Bcr serine/threonine kinase activity is seriously impaired.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
A colorimetric method for determining low concentrations of mercaptans.
TL;DR: A study of the specificity of the reaction indicates that it occurs only with the S − group (actual or potential, as in thiourea), and heavy-metal ions interfere with the reaction by irreversibly bonding any S − ions.
Book ChapterDOI
[66] Pyruvate kinase from muscle: Pyruvate phosphokinase, pyruvic phosphoferase, phosphopyruvate transphosphorylase, phosphate—transferring enzyme II, etc. Phosphoenolpyruvate + ADP ⇌ Pyruvate + ATP
Theodor Bücher,G. Pfleiderer +1 more
Journal ArticleDOI
Crystal structure of cat muscle pyruvate kinase at a resolution of 2.6 A
TL;DR: The structure of pyruvate kinase (EC 2.7.1.40) has been determined from a 2.6 A resolution electron density map and a detailed chain folding to be established for two out of the three domains which make up each of the four identical subunits.
Related Papers (5)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Affinity labeling of rabbit muscle pyruvate kinase by 5'-p-fluorosulfonylbenzoyladenosine.
James L. Wyatt,Roberta F. Colman +1 more