Journal ArticleDOI
Replication of Epstein–Barr virus in human epithelial cells infected in vitro
John W. Sixbey,Ervo H. Vesterinen,John G. Nedrud,Nancy Raab-Traub,Leslie A. Walton,Joseph S. Pagano +5 more
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TLDR
Ability routinely to detect EBV DNA by in situ hybridization in epithelial cells of the oropharynx from persons with acute infectious mononucleosis suggests that, In vivo, EBV regularly gains access to and replicates lytically in epithelium cells.Abstract:
Epstein-Barr virus (EBV), a member of the herpes group of viruses and the aetiological agent of infectious mononucleosis, is usually thought of as a lymphotrophic virus with the ability to transform B lymphocytes. So the association of EBV with nasopharyngeal carcinoma is puzzling, especially given the lack of success of attempts to infect epithelial cells with EBV in culture and the apparent lack of EBV receptors on epithelial cells. Circumvention of the apparent requirement for membrane receptors by techniques of transfection, microinjection and receptor transplantation has clearly demonstrated that there is no inherent barrier to EBV replication in nonlymphoid cells, including epithelial cell types. Our ability routinely to detect EBV DNA by in situ hybridization in epithelial cells of the oropharynx from persons with acute infectious mononucleosis suggests that, in vivo, EBV regularly gains access to and replicates lytically in epithelial cells. We report here in vitro evidence for direct infection by EBV and replication of the virus in cultured normal human epithelial cells.read more
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Journal ArticleDOI
Epstein-Barr Virus Infection
TL;DR: The Epstein–Barr virus (EBV) was discovered 36 years ago by electron microscopy of cells cultured from Burkitt's lymphoma tissue by Epstein, Achong, and Barr and has been found in tissues from other cancers, including T-cell lymphomas and Hodgkin's disease.
Journal ArticleDOI
A novel form of Epstein-Barr virus latency in normal B cells in vivo.
TL;DR: It is concluded that the EBV-infected cells in vivo are B cells with a nonactivated phenotype, which represents a novel form of latency in normal B cells.
Journal ArticleDOI
Replication of Epstein–Barr Virus within the Epithelial Cells of Oral Hairy Leukoplakia, an AIDS-Associated Lesion
John S. Greenspan,Deborah Greenspan,Evelyne T. Lennette,Donald I. Abrams,M.A. Conant,Vibeke Petersen,U K Freese +6 more
TL;DR: It is concluded that EBV replicates within the epithelial cells in hairy leukoplakia, a lesion that is found in immunosuppressed male homosexuals and that is associated with the subsequent development of the acquired immunodeficiency syndrome.
Journal ArticleDOI
Epstein-Barr virus replication in oropharyngeal epithelial cells.
TL;DR: It is concluded that the oropharyngeal epithelial cell may be the target cell type that is productively infected in infectious mononucleosis.
Journal ArticleDOI
A routine method for the establishment of permanent growing lymphoblastoid cell lines
TL;DR: A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Esptein-Barr virus and the immunosuppressivum Cyclosporin A.
References
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Journal ArticleDOI
Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.
Beverley M. Reedman,George Klein +1 more
TL;DR: The ACIF test was used as a tool to trace the Epstein‐Barr virus genome at the cellular level to study the complementfixing antigens of human lymphoblastoid cell lines.
Journal ArticleDOI
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes
TL;DR: Biotin-labeled polynucleotides, both single and double-stranded, are selectively and quantitatively retained on avidin-Sepharose, even after extensive washing with 8 M urea, 6 M guanidine hydrochloride, or 99% formamide.
Journal ArticleDOI
Transformation of foetal human keukocytes in vitro by filtrates of a human leukaemic cell line containing herpes-like virus.
J. H. Pope,M. K. Horne,W. Scott +2 more
TL;DR: Preliminary studies found that the transforming factor was found to be sensitive to heat and ether, was sedimented at 70,000 × g and retained by a filter of APD 50 mμ; these results are compatible with the herpes‐like virus of the QIMR‐WIL cells being the transforming factors.
Journal ArticleDOI
EB viral genomes in epithelial nasopharyngeal carcinoma cells.
TL;DR: Patients with nasopharyngeal carcinomas regularly show high antibody titres directed against Epstein-Barr virus (EBV)-specific antigens, indicating that the incidence of tumours is high in certain populations of South-east Asia, central Africa and Tunisia, but low in most other parts of the world.
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