Journal ArticleDOI
Reprotoxicity of gold, silver, and gold–silver alloy nanoparticles on mammalian gametes
Daniela Tiedemann,Ulrike Taylor,Christoph Rehbock,Jurij Jakobi,Sabine Klein,Wilfried A. Kues,Stephan Barcikowski,Detlef Rath +7 more
TLDR
M mammalian oocytes were sensitive to silver containing nanoparticles, likely, the delicate process of completing meiosis in maternal gametes features high vulnerability towards nanomaterial derived toxicity.Abstract:
Metal and alloy nanoparticles are increasingly developed for biomedical applications, while a firm understanding of their biocompatibility is still missing. Various properties have been reported to influence the toxic potential of nanoparticles. This study aimed to assess the impact of nanoparticle size, surface ligands and chemical composition of gold, silver or gold–silver alloy nanoparticles on mammalian gametes. An in vitro assay for porcine gametes was developed, since these are delicate primary cells, for which well-established culture systems exist and functional parameters are defined. During coincubation with oocytes for 46 h neither any of the tested gold nanoparticles nor the gold–silver alloy particles with a silver molar fraction of up to 50% showed any impact on oocyte maturation. Alloy nanoparticles with 80% silver molar fraction and pure silver nanoparticles inhibited cumulus–oocyte maturation. Confocal microscopy revealed a selective uptake of gold nanoparticles by oocytes, while silver and alloy particles mainly accumulated in the cumulus cell layer surrounding the oocyte. Interestingly sperm vitality parameters (motility, membrane integrity and morphology) were not affected by any of the tested nanoparticles. Only sporadic association of nanoparticles with the sperm plasma membrane was found by transmission electron microscopy. In conclusion, mammalian oocytes were sensitive to silver containing nanoparticles. Likely, the delicate process of completing meiosis in maternal gametes features high vulnerability towards nanomaterial derived toxicity. The results imply that released Ag+-ions are responsible for the observed toxicity, but the compounding into an alloy seemed to alleviate the toxic effects to a certain extent.read more
Citations
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Laser Synthesis and Processing of Colloids: Fundamentals and Applications
TL;DR: Unscreened surface charge of LSPC-synthesized colloids is the key to achieving colloidal stability and high affinity to biomolecules as well as support materials, thereby enabling the fabrication of bioconjugates and heterogeneous catalysts.
Journal ArticleDOI
The oxidative potential of differently charged silver and gold nanoparticles on three human lung epithelial cell types
Paul Schlinkert,Eudald Casals,Matthew S. P. Boyles,Ulrike Tischler,Eva Hornig,Ngoc Tran,Jiayuan Zhao,Martin Himly,Michael Riediker,Gertie Janneke Oostingh,Víctor F. Puntes,Albert Duschl +11 more
TL;DR: Chitosan functionalization of NPs, with resultant high surface charges plays an important role in NP-toxic effects and is dependent on the core material of the particle, the cell type used for testing and the growth characteristics of these cell culture model systems.
Journal ArticleDOI
Applications of Molecularly Imprinted Polymer Nanoparticles and Their Advances toward Industrial Use: A Review
TL;DR: This work is reviewing the numerous applications of molecularly imprinted polymer (MIP) nanoparticles (NPs) and briefly discuss their synthesis, and critically discusses limitations and applications specific problems.
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Synthesis, Structure, Properties, and Applications of Bimetallic Nanoparticles of Noble Metals
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Pulsed laser ablation based synthesis of colloidal metal nanoparticles for catalytic applications.
TL;DR: It is expected that these PLAL-NPs can exhibit higher catalytic activity in comparison to their chemically synthesized counterparts, due to the absence of ligand or stabilizer molecules on the surface of PLAL -metal NPs.
References
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TL;DR: Results of older bio-kinetic studies with NSPs and newer epidemiologic and toxicologic studies with airborne ultrafine particles can be viewed as the basis for the expanding field of nanotoxicology, which can be defined as safety evaluation of engineered nanostructures and nanodevices.
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Andre E. Nel,Lutz Mädler,Darrell Velegol,Tian Xia,Eric M.V. Hoek,Ponisseril Somasundaran,Fred Klaessig,Vince Castranova,Mike Thompson +8 more
TL;DR: Probing the various interfaces of nanoparticle/biological interfaces allows the development of predictive relationships between structure and activity that are determined by nanomaterial properties such as size, shape, surface chemistry, roughness and surface coatings.
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Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles.
Tommy Cedervall,Iseult Lynch,Stina Lindman,Tord Berggård,Eva Thulin,Hanna Nilsson,Kenneth A. Dawson,Sara Linse +7 more
TL;DR: The rates of protein association and dissociation are determined using surface plasmon resonance technology with nanoparticles that are thiol-linked to gold, and through size exclusion chromatography of protein–nanoparticle mixtures, and this method is developed into a systematic methodology to isolate nanoparticle-associated proteins.
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Biomolecular coronas provide the biological identity of nanosized materials
TL;DR: The basic concept of the nanoparticle corona is reviewed and its structure and composition is highlighted, and how the properties of the corona may be linked to its biological impacts are highlighted.
Journal ArticleDOI
Silver as Antibacterial Agent: Ion, Nanoparticle, and Metal
TL;DR: It can be concluded that the therapeutic window for silver is narrower than often assumed, however, the risks for humans and the environment are probably limited.