Journal ArticleDOI
Review and perspectives on microfluidic flow cytometers
TLDR
This review describes some of the major advances made in the microfluidic cytometry field over the past ten years, focusing specifically on recent proposals for enhanced microfluids focusing techniques and detection/analysis methods, respectively.Abstract:
Modern microflow cytometers are sophisticated instruments capable of measuring multiple physical characteristics of a single cell simultaneously as the cells flow in suspension through a measuring device. Cytometers are the tool of choice for the high-speed acquisition and analysis of large single cell populations. However, traditional devices lack the ability to provide intracellular spatial information. In the past few decades, various flow cytometer systems with the ability to combine cell/particle detection and the acquisition of two or three-dimensional spatial information have been proposed. However, these devices suffer a number of drawbacks Accordingly, the problem of developing more sophisticated microflow cytometers based on electrical impedance, optical detection and image analysis methods has received significant attention in the literature. This review describes some of the major advances made in the microfluidic cytometry field over the past ten years. The review focuses specifically on recent proposals for enhanced microfluidic focusing techniques and detection/analysis methods, respectively. Overall, the review provides a useful insight into the microflow cytometer technology field for both new and existing users.read more
Citations
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Journal ArticleDOI
Recent advances in microfluidic cell sorting systems
TL;DR: The merits and demerits of these advanced technologies of sorting are summarized to suggest some suitable combinations of large amount and precise sorting methods to achieve a high-accuracy sorting system with high-throughput.
Journal ArticleDOI
Recent advances in single cell manipulation and biochemical analysis on microfluidics
TL;DR: This review highlights the advances in this field during the past three years in the following three aspects: microfluidic single cell manipulation based on microwells, micropatterns, droplets, traps and flow cytometric methods; detection methods based on fluorescence, mass spectrometry, electrochemical, and polymerase chain reaction-based analysis; and applications in the fields of small molecule detection, protein analysis, multidrug resistance analysis, and single cell sequencing with droplet microflu
Journal ArticleDOI
Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics
TL;DR: A comparative survey of the approaches to elucidate cellular and subcellular features from impedance cytometry data, covering the related subjects of device design, data analytics, and phenotyping applications is presented.
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Microfluidic synthesis control technology and its application in drug delivery, bioimaging, biosensing, environmental analysis and cell analysis
Chia Te Kung,Hongyi Gao,Chia-Yen Lee,Yao Nan Wang,Wenjun Dong,Chien Hsuan Ko,Ge Wang,Lung-Ming Fu,Lung-Ming Fu +8 more
TL;DR: A detailed overview of advances in the microfluidic synthesis technology field over the past five years can be found in this paper, where the authors describe the main microfluidity control mechanisms and micro-fluidity synthesis of particles.
Journal ArticleDOI
Photonic neuromorphic information processing and reservoir computing
Alessio Lugnan,Andrew Katumba,Floris Laporte,Matthias Freiberger,Stijn Sackesyn,Chonghuai Ma,Emmanuel Gooskens,Joni Dambre,Peter Bienstman +8 more
TL;DR: This paper will review some of the exciting work that has been going in this area and then focus on one particular technology, namely, photonic reservoir computing.
References
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Journal ArticleDOI
Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing
TL;DR: The microfabrication and characterisation of an on-chip flow-cytometer is described as the first building block of a complete cell-sorting device and the signal conditioning technique and impedance measurements of cells and particles of different sizes and types are discussed to demonstrate the differentiation of subpopulations in a mixed sample.
Journal ArticleDOI
Real-time deformability cytometry: on-the-fly cell mechanical phenotyping
Oliver Otto,Philipp Rosendahl,Alexander Mietke,Stefan Golfier,Christoph Herold,Daniel Klaue,Salvatore Girardo,Stefano Pagliara,Andrew Ekpenyong,Angela Jacobi,Manja Wobus,Nicole Töpfner,Ulrich F. Keyser,Jörg Mansfeld,Elisabeth Fischer-Friedrich,Jochen Guck +15 more
TL;DR: Real-time deformability cytometry (RT-DC) is introduced for continuous cell mechanical characterization of large populations with analysis rates greater than 100 cells/s and adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
Journal ArticleDOI
Three-dimensional manipulation of single cells using surface acoustic waves
Feng Guo,Zhangming Mao,Yuchao Chen,Zhiwei Xie,James P. Lata,Peng Li,Liqiang Ren,Jiayang Liu,Jian Yang,Ming Dao,Subra Suresh,Tony Jun Huang +11 more
TL;DR: 3D acoustic tweezers are presented, which can trap and manipulate single cells and particles along three mutually orthogonal axes of motion by recourse to surface acoustic waves, and are shown to offer better performance over prior cell manipulation techniques in terms of both accurate and precise motion.
Journal ArticleDOI
Passive mixers in microfluidic systems: A review
TL;DR: Many passive mixers have been proposed to facilitate sample mixing in microfluidic devices over the past five years as mentioned in this paper, and the details of these mixers are briefly described in the following review.
Journal ArticleDOI
High-throughput single-microparticle imaging flow analyzer
Keisuke Goda,Ali Ayazi,Daniel R. Gossett,Daniel R. Gossett,Jagannath Sadasivam,Cejo Konuparamban Lonappan,Elodie Sollier,Elodie Sollier,Ali Fard,Ali Fard,Soojung Claire Hur,Soojung Claire Hur,Jost Adam,Coleman Murray,Chao Wang,Nora Brackbill,Dino Di Carlo,Dino Di Carlo,Bahram Jalali,Bahram Jalali +19 more
TL;DR: An automated flow-through single-particle optical microscope is presented that performs sensitive blur-free image acquisition and nonstop real-time image-recording and classification of microparticles during high-speed flow and demonstrates high-throughput image-based screening of budding yeast and rare breast cancer cells in blood.
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