Rewiring bacteria, two components at a time.
TLDR
A rational method for rewiring the protein-protein interactions and output responses of prokaryotic two-component signal transduction systems has important implications for understanding the specificity of protein interactions and for designing protein-based synthetic signaling cascades.About:
This article is published in Cell.The article was published on 2008-06-13 and is currently open access. It has received 19 citations till now.read more
Citations
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Journal ArticleDOI
Synthesis of orthogonal transcription-translation networks
Wenlin An,Jason W. Chin +1 more
TL;DR: This work demonstrates that system-level dynamic properties are amenable to rational manipulation and design in orthogonal systems and may be further evolved and tuned to provide a spectrum of tailored dynamics in gene expression and investigate the effects of delays in cellular decision-making processes.
Journal ArticleDOI
Engineering Thermostable Microbial Xylanases Toward its Industrial Applications
TL;DR: The present review explores xylanase–substrate interactions using gene-editing tools toward the comprehension in improvement in industrial stability of xylanases.
Journal ArticleDOI
Strain Dependent Genetic Networks for Antibiotic-Sensitivity in a Bacterial Pathogen with a Large Pan-Genome.
TL;DR: The approach uniquely enables genome-wide fitness comparisons across strains, facilitating the discovery that antibiotic responses are complex events that can vary widely between strains, which suggests that in some cases the emergence of resistance could be strain specific and at least for species with a large pan-genome less predictable.
Journal ArticleDOI
Robust signal processing in living cells.
TL;DR: This framework provides a counterpoint to the hypothesis that cellular function relies on an extensive machinery to fine-tune or control intracellular parameters and suggests that for a large class of perturbation, there exists an appropriate topology that renders the network output invariant to the respective perturbations.
Book
The Pangenome: Diversity, Dynamics and Evolution of Genomes
Hervé Tettelin,Duccio Medini +1 more
TL;DR: An early example of Big data in biology: how a mathematical model, developed to address a practical question in vaccinology, transformed established concepts, opening biology to the “unbounded.”
References
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A synthetic oscillatory network of transcriptional regulators
TL;DR: This work used three transcriptional repressor systems that are not part of any natural biological clock to build an oscillating network, termed the repressilator, in Escherichia coli, which periodically induces the synthesis of green fluorescent protein as a readout of its state in individual cells.
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Construction of a genetic toggle switch in Escherichia coli
TL;DR: The construction of a genetic toggle switch is presented—a synthetic, bistable gene-regulatory network—in Escherichia coli and a simple theory is provided that predicts the conditions necessary for bistability.
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Engineering stability in gene networks by autoregulation
Attila Becskei,Luis Serrano +1 more
TL;DR: Simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli are designed and constructed and the gain of stability produced by negative feedback is shown.
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Two-component and phosphorelay signal transduction
TL;DR: Two-component and phosphorelay signal transduction systems are the major means by which bacteria recognize and respond to a variety of environmental stimuli and elucidation of the structures of the interacting domains is leading to understanding of the mechanisms of molecular recognition and phosphotransfer in these systems.
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Engineered riboregulators enable post-transcriptional control of gene expression
Farren J. Isaacs,Daniel J. Dwyer,Chunming Ding,Dmitri D. Pervouchine,Charles R. Cantor,James J. Collins +5 more
TL;DR: A post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression is presented, inserting a complementary cis sequence directly upstream of the ribosome binding site in a target gene.