Role of inoculum and mutant frequency on fosfomycin MIC discrepancies by agar dilution and broth microdilution methods in Enterobacteriaceae.
M. Ballestero-Téllez,Fernando Docobo-Pérez,Fernando Docobo-Pérez,Fernando Docobo-Pérez,José-Manuel Rodríguez-Martínez,José-Manuel Rodríguez-Martínez,José-Manuel Rodríguez-Martínez,M. C. Conejo,M. S. Ramos-Guelfo,Jesús Blázquez,Jesús Blázquez,Jesús Rodríguez-Baño,Álvaro Pascual +12 more
TLDR
The higher inoculum used in the microdilution method enriched the initial inoculum with resistant subpopulations and could partially explain the fosfomycin MIC discrepancies with respect to the agar dilution method.About:
This article is published in Clinical Microbiology and Infection.The article was published on 2017-05-01 and is currently open access. It has received 35 citations till now. The article focuses on the topics: Broth microdilution & Agar dilution.read more
Citations
More filters
Journal ArticleDOI
Mechanisms and clinical relevance of bacterial heteroresistance.
TL;DR: The different genetic mechanisms underlying heterogeneity in antibiotic resistance phenotypes are explored, the impact of such mechanisms on the efficacy of treatment and the methods and difficulties in diagnosing heteroresistance in clinical bacterial isolates are explored.
Journal ArticleDOI
Antibiotic combinations that exploit heteroresistance to multiple drugs effectively control infection.
Victor I. Band,David A. Hufnagel,Siddharth Jaggavarapu,Edgar X. Sherman,Jessie E. Wozniak,Sarah W. Satola,Monica M. Farley,Jesse T. Jacob,Eileen M. Burd,David S. Weiss +9 more
TL;DR: Using carbapenem-resistant Enterobacteriaceae clinical isolates, the underlying basis for the majority of effective combinations to be heteroresistance is revealed, highlighting a rational strategy to identify effective combinations that employs existing antibiotics and could be clinically implemented immediately.
Journal ArticleDOI
Emerging Gram negative resistance to last-line antimicrobial agents fosfomycin, colistin and ceftazidime-avibactam - epidemiology, laboratory detection and treatment implications
TL;DR: Recent developments in resistance to three of the most important last-line antimicrobials for treatment of MDR and XDR Gram negatives: fosfomycin, colistin and ceftazidime-avibactam are described.
Journal ArticleDOI
Azithromycin-loaded respirable microparticles for targeted pulmonary delivery for the treatment of pneumonia
TL;DR: Results demonstrated that AZM@FDKP-MPs administered via intratracheal insufflation achieved at least a 3.4 times higher local concentration and prolonged retention times compared to intravenous injection and oral administration, suggesting their potential to better manage bacterial pneumonia.
Journal ArticleDOI
Frequency and Mechanisms of Spontaneous Fosfomycin Nonsusceptibility Observed upon Disk Diffusion Testing of Escherichia coli.
Aaron E. Lucas,Ryota Ito,Ryota Ito,Mustapha M. Mustapha,Christi L. McElheny,Roberta T. Mettus,Sarah L. Bowler,Serena F. Kantz,Marissa P Pacey,A. William Pasculle,Vaughn S. Cooper,Yohei Doi,Yohei Doi +12 more
TL;DR: Fosfomycin-nonsusceptible inner colony mutants can occur due to the loss of function or induction of UhpT but are rare among multidrug-resistant E. coli clinical strains, considering that these mutants carry high biological costs.
References
More filters
Journal ArticleDOI
The measurement of observer agreement for categorical data
J. R. Landis,Gary G. Koch +1 more
TL;DR: A general statistical methodology for the analysis of multivariate categorical data arising from observer reliability studies is presented and tests for interobserver bias are presented in terms of first-order marginal homogeneity and measures of interob server agreement are developed as generalized kappa-type statistics.
Journal ArticleDOI
International clinical practice guidelines for the treatment of acute uncomplicated cystitis and pyelonephritis in women: A 2010 update by the Infectious Diseases Society of America and the European Society for Microbiology and Infectious Diseases
Kalpana Gupta,Thomas M. Hooton,Kurt G. Naber,Richard Colgan,Loren G. Miller,Gregory J. Moran,Lindsay E. Nicolle,Raul Raz,Anthony J. Schaeffer,David E. Soper,Miami Florida +10 more
TL;DR: Treatment of women with acute uncomplicated cystitis and pyelonephritis is limited in these guidelines to premenopausal, non-pregnant women with no known urological abnormalities or co-morbidities.
Journal ArticleDOI
Comparing methods of measurement: why plotting difference against standard method is misleading
J M Bland,Douglas G. Altman +1 more
TL;DR: A plot of the difference against the average of the standard and new measurements is unlikely to mislead in this way and is shown theoretically and by a practical example.
Journal ArticleDOI
Pneumonia Caused by Methicillin-Resistant Staphylococcus aureus
TL;DR: Clinical features of, diagnosis of, and therapies for MRSA pneumonia are reviewed, including linezolid as an alternative to vancomycin, and Respiratory infection-control measures and de-escalation of initial broad-spectrum antibiotic regimens to avoid emergence of resistant organisms are reviewed.