Journal ArticleDOI
Secondary metabolism in micropropagated Hypericum perforatum L. grown in non-aerated liquid medium
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TLDR
A system that could lower the cost of in vitro propagation by using liquid medium, as well as to evaluate the secondary metabolism in the systems tested, was established.Abstract:
Hypericum perforatum L. is a medicinal plant that has been extensively studied because of its bioactive properties. The objective of this study was to establish a system that could lower the cost of in vitro propagation by using liquid medium, as well as to evaluate the secondary metabolism in the systems tested. Nodal segments of H. perforatum were obtained from in vitro shoots and grown in three liquid culture systems: total immersion (TI), partial immersion (PI), and paper bridge support (PB). Semi-solid medium (3 g L−1 Phytagel™) was used as control (SS). The organogenic responses were evaluated, and phenolic compounds, hypericin, and the activity of polyphenol oxidases (PPO) and peroxidases (POX) were quantified. After 80 days of culture, induction and proliferation of adventitious shoots were similar in the PI and SS systems (65.3 and 71.3 shoots, respectively), whereas PB resulted in the fewest shoots per explant (29.5 shoots). Longer shoots were obtained under the PI conditions. Hyperhydricity was observed in the shoots from the TI system. Browning was visible in shoots from the TI and PB systems. The highest concentrations of phenolic compounds and hypericin were observed in shoots derived from PI and PB, at 80 days of culture. POX activity was higher in shoots cultured in PI at 40 days, whereas PPO was significantly more active at 80 days of culture. Likely, POX was more related to shoot growth, whereas PPO played a later role in response to the culture environment and medium stress.read more
Citations
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Stress and defense responses in plant secondary metabolites production
TL;DR: Application of molecular biology tools and techniques are facilitating understanding the signaling processes and pathways involved in the SMs production at subcellular, cellular, organ and whole plant systems during in vivo and in vitro growth, with application in metabolic engineering of biosynthetic pathways intermediates.
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In vitro plant regeneration, secondary metabolite production and antioxidant activity of micropropagated Aloe arborescens Mill
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Journal ArticleDOI
The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatum L.
Sonia Gadzovska,Stéphane Maury,Stéphane Maury,Alain Delaunay,Alain Delaunay,Mirko Spasenoski,Daniel Hagège,Didier Courtois,Claude Joseph,Claude Joseph +9 more
TL;DR: The H. perforatum cells were globally more sensitive to salicylic acid elicitation when maintained in an undifferentiated state and particularly in cell suspension cultures, implying that salcylic acid could act at biosynthesis level but not for the accumulation of both hypericin and pseudohypericin.
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Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum
TL;DR: The activities of catalase (CAT) were inhibited after MeJA treatment in the cell cultures, while the activities of phenylalanine ammonia lyase (PAL) increased, which led to the enhancement of flavonoid production.
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Photoautotrophic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]
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TL;DR: The increase of 20E levels by the photoautotrophic system offers new prospects for increasing the commercial production of this species, and for studies that could elucidate the biosynthetic pathway of phytoecdysteroids in plants.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts
TL;DR: The antioxidant potentials of a total ethanolic extract of Hypericum perforatum (TE) and fractions were evaluated and correlated with their phenolic contents in this article, and the extracts were fully characterised by HPLC-DAD-MS-MS.
Journal ArticleDOI
Hypericins as potential leads for new therapeutics.
TL;DR: The aim of this review is to analyse the most recent advances in the chemistry and biological activities (in vitro and in vivo) of the pure naphthodianthrones, hypericin and pseudohypericin from H. perforatum.
Journal ArticleDOI
Application of bioreactor systems for large scale production of horticultural and medicinal plants
TL;DR: Low cost and less labour-intensive clonal propagation through the use of modified air-lift, bubble column, bioreactors, together with temporary immersion systems for the propagation of shoots, bud-clusters and somatic embryos is described.
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