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Open AccessJournal ArticleDOI

Separation of Submicron Bioparticles by Dielectrophoresis

Hywel Morgan, +2 more
- 01 Jul 1999 - 
- Vol. 77, Iss: 1, pp 516-525
TLDR
With electrode arrays fabricated using direct write electron beam lithography, it is shown that different types of submicron latex spheres can be spatially separated and it is demonstrated that tobacco mosaic virus and herpes simplex virus can be manipulated and spatially separation in a microelectrode array.
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This article is published in Biophysical Journal.The article was published on 1999-07-01 and is currently open access. It has received 533 citations till now. The article focuses on the topics: Dielectrophoresis & Particle.

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Citations
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Journal ArticleDOI

Experimental analysis of particle and fluid motion in ac electrokinetics

TL;DR: In this article, a two-color particle image velocimetry (μ-PIV) was developed and used to uniquely determine the fluid velocity from the observation of particles without a priori knowledge of the electrical properties.
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Trapping of 27 bp–8 kbp DNA and immobilization of thiol-modified DNA using dielectrophoresis

TL;DR: The immobilization of trapped hexanethiol- and DTPA-modified 140 nm long DNA to the end of gold nanoelectrodes was experimentally quantified and the observations were supported by density functional theory calculations.
Journal ArticleDOI

Fractionation and characterization of nano- and microparticles in liquid media.

TL;DR: The review is focused on the methods for fractionation and characterization of nanoparticles and microparticles in liquid media, the most representative examples of their application, and the trends in developing novel approaches to the separation and investigation of particles.
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Extraction of dielectric properties of multiple populations from dielectrophoretic collection spectrum data.

TL;DR: The observation of the frequencies of onset of dielectric dispersion allows the identification and enumeration of populations of cells according to cytoplasmic conductivity, with particular relevance to the determination of the action of drugs for high-throughput screening applications.
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Bacteria concentration using a membrane type insulator-based dielectrophoresis in a plastic chip.

TL;DR: Simulations using CFD‐ACE+(CFD Research, Huntsville, Alabama) suggested that the dielectrophoresis force is stronger in the edge of the pores where the field gradient is highest.
References
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Book

CRC Handbook of Chemistry and Physics

TL;DR: CRC handbook of chemistry and physics, CRC Handbook of Chemistry and Physics, CRC handbook as discussed by the authors, CRC Handbook for Chemistry and Physiology, CRC Handbook for Physics,
Book

Electromechanics of Particles

TL;DR: In this paper, a detailed account of the electromechanical interactions that govern the behaviour of small particles when an electric or magnetic field is present is presented, with numerous real-world examples.
Journal ArticleDOI

Ac electrokinetics: a review of forces in microelectrode structures

TL;DR: In this article, the frequency dependency and magnitude of electrothermally induced fluid flow are discussed for low frequencies (up to 500 kHz) and the effects of Brownian motion, diffusion and buoyancy force are discussed in the context of the controlled manipulation of sub-micrometre particles.
Journal ArticleDOI

The Motion and Precipitation of Suspensoids in Divergent Electric Fields

TL;DR: In this article, it was shown that the phenomena of dielectrophoresis and dielectro-precipitation can be observed under rather ideal conditions, though it is ordinarily often difficult to do so because of the presence of the more easily produced electrophoreis or ion-type migration of charged particles.
Journal ArticleDOI

Separation of Human Breast Cancer Cells From Blood by Differential Dielectric Affinity

TL;DR: Findings indicate that the dielectric affinity technique may prove useful in a wide variety of cell separation and characterization applications.
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